Ssu72 protein mediates both poly(A)-coupled and poly(A)-independent termination of RNA polymerase II transcription

被引:100
作者
Steinmetz, EJ [1 ]
Brow, DA [1 ]
机构
[1] Univ Wisconsin, Sch Med, Dept Biomol Chem, Madison, WI 53706 USA
关键词
D O I
10.1128/MCB.23.18.6339-6349.2003
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Termination of transcription by RNA polymerase 11 (Pol 11) is a poorly understood yet essential step in eukaryotic gene expression. Termination of pre-mRNA synthesis is coupled to recognition of RNA signals that direct cleavage and polyadenylation of the nascent transcript. Termination of nonpolyadenylated transcripts made by Pol 11 in the yeast Saccharomyces cerevisiae, including the small nuclear and small nucleolar RNAs, requires distinct RNA elements recognized by the Nrd1 protein and other factors. We have used genetic selection to characterize the terminator of the SNR13 snoRNA gene, revealing a bipartite structure consisting of an upstream element closely matching a Nrd1-binding sequence and a downstream element similar to a cleavage/polyadenylation signal. Genome-wide selection for factors influencing recogniton of the SNR13 terminator yielded mutations in the gene coding for the essential Pol II-binding protein Ssu72. Ssu72 has recently been found to associate with the pre-mRNA cleavage/polyadenylation machinery, and we find that an ssu72 mutation that disrupts Nrd1-dependent termination also results in deficient poly(A) -dependent termination. These findings extend the parallels between the two termination pathways and suggest that they share a common mechanism to signal Pol 11 termination.
引用
收藏
页码:6339 / 6349
页数:11
相关论文
共 58 条
[11]   Opposing effects of Ctk1 kinase and Fcp1 phosphatase at Ser 2 of the RNA polymerase II C-terminal domain [J].
Cho, EJ ;
Kobor, MS ;
Kim, M ;
Greenblatt, J ;
Buratowski, S .
GENES & DEVELOPMENT, 2001, 15 (24) :3319-3329
[12]  
COLLART MA, 1993, CURRENT PROTOCOLS S, V23
[13]   A FUNCTIONAL MESSENGER-RNA POLYADENYLATION SIGNAL IS REQUIRED FOR TRANSCRIPTION TERMINATION BY RNA POLYMERASE-II [J].
CONNELLY, S ;
MANLEY, JL .
GENES & DEVELOPMENT, 1988, 2 (04) :440-452
[14]  
Conrad NK, 2000, GENETICS, V154, P557
[15]   SEN1, A POSITIVE EFFECTOR OF TRANSFER RNA-SPLICING ENDONUCLEASE IN SACCHAROMYCES-CEREVISIAE [J].
DEMARINI, DJ ;
WINEY, M ;
URSIC, D ;
WEBB, F ;
CULBERTSON, MR .
MOLECULAR AND CELLULAR BIOLOGY, 1992, 12 (05) :2154-2164
[16]   Yhh1p/Cft1p directly links poly(A) site recognition and RNA polymerase II transcription termination [J].
Dichtl, B ;
Blank, D ;
Sadowski, M ;
Hübner, W ;
Weiser, S ;
Keller, W .
EMBO JOURNAL, 2002, 21 (15) :4125-4135
[17]   A role for SSU72 in balancing RNA polymerase II transcription elongation and termination [J].
Dichtl, B ;
Blank, D ;
Ohnacker, M ;
Friedlein, A ;
Roeder, D ;
Langen, H ;
Keller, W .
MOLECULAR CELL, 2002, 10 (05) :1139-1150
[18]   Yeast snoRNA accumulation relies on a cleavege-dependent/polyadenylation-independent 3′-processing apparatus [J].
Fatica, A ;
Morlando, M ;
Bozzoni, I .
EMBO JOURNAL, 2000, 19 (22) :6218-6229
[19]   Ssu72 is a phosphatase essential for transcription termination of snoRNAs and specific mRNAs in yeast [J].
Ganem, C ;
Devaux, F ;
Torchet, C ;
Jacq, C ;
Quevillon-Cheruel, S ;
Labesse, G ;
Facca, C ;
Faye, G .
EMBO JOURNAL, 2003, 22 (07) :1588-1598
[20]   Functional organization of the yeast proteome by systematic analysis of protein complexes [J].
Gavin, AC ;
Bösche, M ;
Krause, R ;
Grandi, P ;
Marzioch, M ;
Bauer, A ;
Schultz, J ;
Rick, JM ;
Michon, AM ;
Cruciat, CM ;
Remor, M ;
Höfert, C ;
Schelder, M ;
Brajenovic, M ;
Ruffner, H ;
Merino, A ;
Klein, K ;
Hudak, M ;
Dickson, D ;
Rudi, T ;
Gnau, V ;
Bauch, A ;
Bastuck, S ;
Huhse, B ;
Leutwein, C ;
Heurtier, MA ;
Copley, RR ;
Edelmann, A ;
Querfurth, E ;
Rybin, V ;
Drewes, G ;
Raida, M ;
Bouwmeester, T ;
Bork, P ;
Seraphin, B ;
Kuster, B ;
Neubauer, G ;
Superti-Furga, G .
NATURE, 2002, 415 (6868) :141-147