Behavior of ectopic surface:: effects of β-adrenergic stimulation and uncoupling

被引:16
作者
Arutunyan, A
Pumir, A
Krinsky, V
Swift, L
Sarvazyan, N
机构
[1] Texas Tech Univ, Hlth Sci Ctr, Dept Physiol, Lubbock, TX 79430 USA
[2] CNRS, Inst Nonlineaire Nice, F-06560 Valbonne, France
来源
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY | 2003年 / 285卷 / 06期
关键词
zone; cardiac arrhythmia; functional border zone; ischemia-reperfusion injury;
D O I
10.1152/ajpheart.00381.2003
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
By using both experimental and theoretical means, we have addressed the progression of ectopic activity from individual cardiac cells to a multicellular two-dimensional network. Experimental conditions that favor ectopic activity have been created by local perfusion of a small area of cardiomyocyte network (I-zone) with an isoproterenol-heptanol containing solution. The application of this solution initially slowed down and then fully blocked wave propagation inside the I-zone. After a brief lag period, ectopically active cells appeared in the I-zone, followed by evolution of the ectopic clusters into slowly propagating waves. The changing pattern of colliding and expanding ectopic waves confined to the I-zone persisted for as long as the isoproterenol-heptanol environment was present. On restoration of the control environment, the ectopic waves from the I-zone broke out into the surrounding network causing arrhythmias. The observed sequence of events was also modeled by FitzHugh-Nagumo equations and included a cell's arrangement of two adjacent square regions of 20 x 20 cells. The control zone consisted of well-connected, excitable cells, and the I-zone was made of weakly coupled cells ( heptanol effect), which became spontaneously active as time evolved ( isoproterenol effect). The dynamic events in the system have been studied numerically with the use of a finite difference method. Together, our experimental and computational data have revealed that the combination of low coupling, increased excitability, and spatial heterogeneity can lead to the development of ectopic waves confined to the injured network. This transient condition appears to serve as an essential step for the ectopic activity to "mature" before escaping into the surrounding control network.
引用
收藏
页码:H2531 / H2542
页数:12
相关论文
共 39 条
  • [11] Cardiac ionic currents and acute ischemia: From channels to arrhythmias
    Carmeliet, E
    [J]. PHYSIOLOGICAL REVIEWS, 1999, 79 (03) : 917 - 1017
  • [12] Cellular uncoupling during ischemia in hypertrophied and failing rabbit ventricular myocardium - Effects of preconditioning
    Dekker, LRC
    Rademaker, H
    Vermeulen, JT
    Opthof, T
    Coronel, R
    Spaan, JAE
    Janse, MJ
    [J]. CIRCULATION, 1998, 97 (17) : 1724 - 1730
  • [13] An integrative model of the cardiac ventricular myocyte incorporating local control of Ca2+ release
    Greenstein, JL
    Winslow, RL
    [J]. BIOPHYSICAL JOURNAL, 2002, 83 (06) : 2918 - 2945
  • [14] A spontaneously active focus drives a model atrial sheet more easily than a model ventricular sheet
    Joyner, RW
    Wang, YG
    Wilders, R
    Golod, DA
    Wagner, MB
    Kumar, R
    Goolsby, WN
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2000, 279 (02): : H752 - H763
  • [15] PROPAGATION THROUGH ELECTRICALLY COUPLED CELLS - HOW A SMALL SA NODEDRIVES A LARGE ATRIUM
    JOYNER, RW
    VANCAPELLE, FJL
    [J]. BIOPHYSICAL JOURNAL, 1986, 50 (06) : 1157 - 1164
  • [16] Keener J. P., 1998, MATH PHYSL
  • [17] REVERSIBLE INHIBITION OF GAP JUNCTIONAL INTERCELLULAR COMMUNICATION, SYNCHRONOUS CONTRACTION, AND SYNCHRONISM OF INTRACELLULAR CA2+ FLUCTUATION IN CULTURED NEONATAL RAT CARDIAC MYOCYTES BY HEPTANOL
    KIMURA, H
    OYAMADA, Y
    OHSHIKA, H
    MORI, M
    OYAMADA, N
    [J]. EXPERIMENTAL CELL RESEARCH, 1995, 220 (02) : 348 - 356
  • [18] The gap junction communication channel
    Kumar, NM
    Gilula, NB
    [J]. CELL, 1996, 84 (03) : 381 - 388
  • [19] Experimental model for an ectopic focus coupled to ventricular cells
    Kumar, R
    Wilders, R
    Joyner, RW
    Jongsma, HJ
    Verheijck, EE
    Golod, DA
    vanGinneken, ACG
    Goolsby, WN
    [J]. CIRCULATION, 1996, 94 (04) : 833 - 841
  • [20] BETA(2)-ADRENERGIC RECEPTOR ACTIONS IN NEONATAL AND ADULT-RAT VENTRICULAR MYOCYTES
    KUZNETSOV, V
    PAK, E
    ROBINSON, RB
    STEINBERG, SF
    [J]. CIRCULATION RESEARCH, 1995, 76 (01) : 40 - 52