Requirement of transcription factor AML1 in proliferation of developing thymocytes

Although the transcription factor AML1/Runx1 is known to be essential for definitive hematopoiesis, its role in T cell differentiation is not well understood. In this study, we investigated the functions of AML1 in the early stage of thymocyte differentiation. For this, we crossed AML1 dominant interfering form (Runt)-transgenic mice with TCR-transgenic mice, and demonstrated the decrease of CD4(+)8(+) (DP) thymocyte cell number although their proportion was not reduced. Reaggregation culture system for thymocytes of (Runt x TCR) double transgenic mice, in which the rate of de novo transition from DN cells to the DP stage can be estimated, showed that the cell division during the DN-to-DP transition is impaired without significant cell death. These results indicate that AMLI is involved in thymocyte differentiation by controlling cell proliferation. (C) 2003 Elsevier Science B.V. All rights reserved.