Some features of intestinal absorption of intact fibrinolytic enzyme III-1 from Lumbricus rubellus

被引：49

作者：

Fan, Q ^{[1
]}

Wu, C ^{[1
]}

Li, L ^{[1
]}

Fan, R ^{[1
]}

Wu, C ^{[1
]}

Hou, QM ^{[1
]}

He, RQ ^{[1
]}

机构：

[1] Chinese Acad Sci, Baiao Pharmaceut Beijing CL, Inst Biophys, Lab Visual Informat Proc, Beijing 100101, Peoples R China

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 2001年 / 1526卷 / 03期

关键词：

earthworm fibrinolytic enzyme; protease; horseradish peroxidase; intestinal absorption; intraperitoneal injection; immunohistochemistry; immunoblotting; ELISA;

D O I：

10.1016/S0304-4165(01)00140-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to investigate whether earthworm fibrinolytic enzyme III-1 (EFE-III-1) isolated from Lumbricus rubellus is capable of transporting into blood through intestinal epithelium and keeping its biological function in circulation, we have raised an antibody against EFE-III-I. The immunological results showed that 10-15% of intact EFE-III-I was absorbed by the intestinal epithelium with the incubation chamber method [Vilhardt and Lundin, Acta Physiol. Scand. 126 (1986) 601-607]. The enzyme could be detected in the intestinal epithelial cells by immunohistochemistry. Furthermore, immunoreactive intact EFE-III-I was found in serum or plasma after intraperitoneal injection of rats. Approx. 10% of the full-size enzyme could transport through the intestinal epithelium. The maximum remaining activity in blood could be assayed around 60 min after the intraperitoneal injection. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：286 / 292

页数：7

共 29 条

[1] AN OLIGOPEPTIDE PERMEATES INTESTINAL TIGHT JUNCTIONS AT GLUCOSE-ELICITED DILATATIONS - IMPLICATIONS FOR OLIGOPEPTIDE ABSORPTION [J].