Separation and free radical-scavenging activity of major curcuminoids of Curcuma longa using HPTLC-DPPH method

被引：60

作者：

Pozharitskaya, Olga N. ^{[1
]}

Ivanova, Svetlana A. ^{[1
]}

Shikov, Alexander N. ^{[1
]}

Makarov, Valery G. ^{[1
]}

机构：

[1] Interreg Ctr Adaptogen, St Petersburg 195067, Russia

来源：

PHYTOCHEMICAL ANALYSIS | 2008年 / 19卷 / 03期

关键词：

1,1-Diphenyl-2-picrythydrazyl; Curcurna longa; curcummoids; free radical-scavenging activity; HPTLC;

D O I：

10.1002/pca.1035

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A direct HPTLC assay was developed for the determination of total curcummoids and three individual curcummoids, curcumin. demethoxycurcumin and bisdemethoxycurcumin. In addition, a new procedure was developed to separate and quantitative the free radical-scavenging activity of individual compounds from the rhizome of Curcuma longa L. (Zingiberaceae) based on the combination of HPTLC with a diode array detector (DAD) and post chromatographic DPPH* radical derivatisation. It was established that both individual curcummoids and the extract of C. longa were capable of scavenging DPPH* radicals. From the estimated ID50 values, it can be seen that the order of activity was curcumin > demethoxycurcumin > bisdemethoxycurcumin >> ascorbic acid. However, the ID50 values of curcuminoids were not significantly different. The data indicates the presence of a synergistic mechanism of antiradical activity of curcummoids. Copyright (c) 2007 John Wiley & Sons, Ltd.

引用

页码：236 / 243

页数：8

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