Isolation of mutants of Penicillium purpurogenum resistant to catabolite repression

被引:22
作者
Anwar, MN
Suto, M
Tomita, F
机构
[1] HOKKAIDO UNIV,FAC AGR,DEPT BIOSCI & CHEM,APPL MICROBIOL LAB,SAPPORO,HOKKAIDO 060,JAPAN
[2] CHITTAGONG UNIV,DEPT BOT,CHITTAGONG 4331,BANGLADESH
关键词
D O I
10.1007/s002530050748
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The strain Penicillium purpurogenum P-26 was subjected to UV irradiation and N-methyl-N'-nitro-N-nitrsoguanidine treatment and mutants were isolated capable of synthesizing cellulase under the conditions of a high concentration of glucose, Initially mutants resistant to catabolite repression by 2-deoxy-D-glucose were isolated on Walseth's cellulose/agar plates containing 15-45 mM 2-deoxy-D-glucose. These mutants were again screened for resistance to clatabolite repression by glycerol or glucose on Walseth's cellulose/agar plates containing 50 g/l glycerol or 50 g/l glucose respectively. Four mutants with different sizes of clearing zone on Walseth's cellulose/agar plates containing 50 g/l glucose were selected for flask culture. Among them, the mutant NTUV-45-4 showed better carboxymethylcellulase activity in flask culture containing 1% Avicel plus 3% glucose than did the parental strain.
引用
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页码:684 / 687
页数:4
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