Isolation of mutants of Penicillium purpurogenum resistant to catabolite repression

被引：22

作者：

Anwar, MN

Suto, M

Tomita, F

机构：

[1] HOKKAIDO UNIV,FAC AGR,DEPT BIOSCI & CHEM,APPL MICROBIOL LAB,SAPPORO,HOKKAIDO 060,JAPAN

[2] CHITTAGONG UNIV,DEPT BOT,CHITTAGONG 4331,BANGLADESH

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1996年 / 45卷 / 05期

关键词：

D O I：

10.1007/s002530050748

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The strain Penicillium purpurogenum P-26 was subjected to UV irradiation and N-methyl-N'-nitro-N-nitrsoguanidine treatment and mutants were isolated capable of synthesizing cellulase under the conditions of a high concentration of glucose, Initially mutants resistant to catabolite repression by 2-deoxy-D-glucose were isolated on Walseth's cellulose/agar plates containing 15-45 mM 2-deoxy-D-glucose. These mutants were again screened for resistance to clatabolite repression by glycerol or glucose on Walseth's cellulose/agar plates containing 50 g/l glycerol or 50 g/l glucose respectively. Four mutants with different sizes of clearing zone on Walseth's cellulose/agar plates containing 50 g/l glucose were selected for flask culture. Among them, the mutant NTUV-45-4 showed better carboxymethylcellulase activity in flask culture containing 1% Avicel plus 3% glucose than did the parental strain.

引用

页码：684 / 687

页数：4