Early tissue response to citric acid-based micro- and nanocomposites

被引:29
作者
Chung, Eun Ji [1 ]
Qiu, Hongjin [1 ]
Kodali, Pradeep [2 ]
Yang, Scott [2 ]
Sprague, Stuart M. [3 ,4 ]
Hwong, James [4 ]
Koh, Jason [2 ]
Ameer, Guillermo A. [1 ,5 ,6 ]
机构
[1] Northwestern Univ, Dept Biomed Engn, Evanston, IL 60208 USA
[2] NW Mem Hosp, Dept Orthopaed Surg, Chicago, IL 60611 USA
[3] Northshore Univ HealthSyst, Evanston, IL 60201 USA
[4] Northwestern Univ, Feinberg Sch Med, Chicago, IL 60611 USA
[5] Northwestern Univ, Dept Surg, Chicago, IL 60611 USA
[6] Northwestern Univ, Inst BioNanotechnol Med, Chicago, IL 60611 USA
关键词
bone tissue engineering; calcium phosphate; hydroxyapatite; cartilage tissue engineering; tissue interface; L-LACTIC-ACID; CRUCIATE LIGAMENT RECONSTRUCTION; MECHANICAL-PROPERTIES; COMPOSITE SCAFFOLDS; IN-VIVO; HYDROXYAPATITE COMPOSITE; BIODEGRADABLE ELASTOMERS; MEDICAL APPLICATIONS; ORTHOPEDIC IMPLANTS; OSTEOBLAST ADHESION;
D O I
10.1002/jbm.a.32953
中图分类号
R318 [生物医学工程];
学科分类号
100103 [病原生物学];
摘要
Composites based on calcium phosphates and biodegradable polymers are desirable for orthopedic applications because of their potential to mimic bone. Herein, we describe the fabrication, characterization, and in vivo response of novel citric acid-based microcomposites and nanocomposites. Poly(1,8-octanediol-co-citrate) (POC) was mixed with increasing amounts of hydroxyapatite (HA) nanoparticles or microparticles (up to 60 wt %), and the morphology and mechanical properties of the resulting composites were assessed. To investigate tissue response, nanocomposites, microcomposites, POC, and poly(L-lactide) were implanted in osteochondral defects in rabbits and harvested at 6 weeks for histological evaluation. Scanning electron microscopy confirmed increased surface roughness of microcomposites relative to nanocomposites. The mechanical properties of both types of composites increased with increasing amounts of HA (8-328 MPa), although nanocomposites with 60 wt % HA displayed the highest strength and stiffness. Based on tissue-implant interfacial assessments, all implants integrated well with the surrounding bone and cartilage with no evidence of inflammation. Both nanocomposites and microcomposites supported bone remodeling; however, nanocomposites induced more trabecular bone formation at the tissue-implant interface. The mechanical properties of citric acid-based composites are within the range of human trabecular bone (1-1524 MPa, 211 +/- 78 MPa mean modulus), and tissue response was dependent on the size and content of HA, providing new perspectives of design and fabrication criteria for orthopedic devices such as interference screws and fixation pins. (C) 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 96A: 29-37, 2011.
引用
收藏
页码:29 / 37
页数:9
相关论文
共 65 条
[1]
Tensile properties, tension-tension fatigue and biological response of polyetheretherketone-hydroxyapatite composites for load-bearing orthopedic implants [J].
Abu Bakar, MS ;
Cheng, MHW ;
Tang, SM ;
Yu, SC ;
Liao, K ;
Tan, CT ;
Khor, KA ;
Cheang, P .
BIOMATERIALS, 2003, 24 (13) :2245-2250
[2]
A biomechanical comparison of Bosworth and poly-L lactic acid bioabsorbable screws for treatment of acromioclavicular separations [J].
Ammon, JT ;
Voor, MJ ;
Tillett, ED .
ARTHROSCOPY-THE JOURNAL OF ARTHROSCOPIC AND RELATED SURGERY, 2005, 21 (12) :1443-1446
[3]
Effect of blending calcium compounds on hydrolytic degradation of poly(DL-lactic acid-co-glycolic acid) [J].
Ara, M ;
Watanabe, M ;
Imai, Y .
BIOMATERIALS, 2002, 23 (12) :2479-2483
[4]
Nanotechnology and biomaterials for orthopedic medical applications [J].
Balasundaram, Ganesan ;
Webster, Thomas J. .
NANOMEDICINE, 2006, 1 (02) :169-176
[5]
LATE DEGRADATION TISSUE-RESPONSE TO POLY(L-LACTIDE) BONE PLATES AND SCREWS [J].
BERGSMA, JE ;
DEBRUIJN, WC ;
ROZEMA, FR ;
BOS, RRM ;
BOERING, G .
BIOMATERIALS, 1995, 16 (01) :25-31
[6]
COMPRESSIVE BEHAVIOR OF BONE AS A 2-PHASE POROUS STRUCTURE [J].
CARTER, DR ;
HAYES, WC .
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 1977, 59 (07) :954-962
[7]
Composite Chitosan/Nano-Hydroxyapatite Scaffolds Induce Osteocalcin Production by Osteoblasts In Vitro and Support Bone Formation In Vivo [J].
Chesnutt, Betsy M. ;
Yuan, Youling ;
Buddington, Karyl ;
Haggard, Warren O. ;
Bumgardner, Joel D. .
TISSUE ENGINEERING PART A, 2009, 15 (09) :2571-2579
[8]
Modulation of bone ingrowth of rabbit femur titanium implants by in vivo axial micromechanical loading [J].
Clark, PA ;
Rodriguez, A ;
Sumner, DR ;
Hussain, MA ;
Mao, JJ .
JOURNAL OF APPLIED PHYSIOLOGY, 2005, 98 (05) :1922-1929
[9]
COOKE FW, 1992, CLIN ORTHOP RELAT R, P135
[10]
Polymer nanocomposites: The "nano" effect on mechanical properties [J].
Crosby, Alfred J. ;
Lee, Jong-Young .
POLYMER REVIEWS, 2007, 47 (02) :217-229