On the interaction of [Ru(phen)2dppz]2+ (dppz = dipyrido[3,2-a:2′,3′-c]phenazine) with different oligonucleotides

被引：48

作者：

Nair, RB ^{[1
]}

Murphy, CJ ^{[1
]}

机构：

[1] Univ S Carolina, Dept Chem & Biochem, Columbia, SC 29208 USA

来源：

JOURNAL OF INORGANIC BIOCHEMISTRY | 1998年 / 69卷 / 1-2期

基金：

美国国家科学基金会;

关键词：

oligonucleotides; Ru; dppz; DNA probe;

D O I：

10.1016/S0162-0134(97)10033-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Luminescent titrations have been performed on a "molecular light switch for DNA", [Ru(phen)(2)dppz](2+) (dppz = dipyrido[3,2-a:2',3'-c]phenazine), with several different 16-bp oligonucleotides having sequence-directed shape ("straight", "bent" and "kinked"). Binding constants and binding site sizes are consistent with metal complex intercalation for all oligonucleotides. The emission maxima and relative intensities upon saturation are consistent with trends found for long DNAs based on relative GC content. [Ru(phen)2dppz]2' has a significantly higher binding constant and larger site size for the "kinked" oligonucleotide than for the "straight" and "bent", suggesting that local DNR deformation plays a role in the interaction of metallointercalators with DNA. (C) 1998 Elsevier Science Inc. All rights reserved.

引用

页码：129 / 133

页数：5

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