A new hydroxylase system in Actinomadura sp cells converting compactin to pravastatin

被引：22

作者：

Peng, Y ^{[1
]}

Demain, AL ^{[1
]}

机构：

[1] MIT, Dept Biol, Fermentat Microbiol Lab, Cambridge, MA 02139 USA

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY | 1998年 / 20卷 / 06期

关键词：

Actinomadura; compactin; hydroxylase; microbial transformation; pravastatin;

D O I：

10.1038/sj.jim.2900539

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The hydroxylase in cell-free extracts of Actinomadura sp strain 2966 converts compactin to pravastatin, It requires NADPH as coenzyme and Mg2+ as cofactor; Mn2+ can partially replace Mg2+. In contrast with the inducible cytochrome P-450 system of Streptomyces carbophilus which catalyzes the same overall reaction, this constitutive hydroxylase is stimulated by ATP and ascorbic acid and is not inactivated by CO.

引用

页码：373 / 375

页数：3

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