Recombinant spider silk as matrices for cell culture

被引：110

作者：

Widhe, Mona ^{[1
]}

Bysell, Helena ^{[2
]}

Nystedt, Sara ^{[3
]}

Schenning, Ingrid ^{[3
]}

Malmsten, Martin ^{[2
]}

Johansson, Jan ^{[1
]}

Rising, Anna ^{[1
]}

Hedhammar, My ^{[1
]}

机构：

[1] Swedish Univ Agr Sci, Dept Anat Physiol & Biochem, Biomed Ctr, S-75123 Uppsala, Sweden

[2] Uppsala Univ, Dept Pharm, Biomed Ctr, S-75123 Uppsala, Sweden

[3] Spiber Technol AB, Uppsala, Sweden

来源：

BIOMATERIALS | 2010年 / 31卷 / 36期

基金：

瑞典研究理事会;

关键词：

Biomaterial; Cytocompatibility; Collagen production; Fibroblasts; Serum free; Tissue engineering; EMBRYONIC STEM-CELLS; TERM SELF-RENEWAL; WETTABILITY GRADIENT; 3; DIMENSIONS; POLYMER; FIBERS; FIBROBLASTS; SCAFFOLDS; RESPONSES; SURFACES;

D O I：

10.1016/j.biomaterials.2010.08.061

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

The recombinant miniature spider silk protein 4RepCT was used to fabricate film foam fiber and mesh matrices of different dimensionality microstructure and nanotopography These matrices were evaluated regarding their suitability for cell culturing Human primary fibroblasts attached to and grew well on all matrix types also in the absence of serum proteins or other animal-derived additives The highest cell counts were obtained on matrices combining film and fiber/mesh The cells showed an elongated shape that followed the structure of the matrices and exhibited prominent actin filaments Moreover the fibroblasts produced secreted and deposited collagen type I onto the matrices These results together with findings of the matrices being mechanically robust hold promise not only for in vitro cell culturing but also for tissue engineering applications (C) 2010 Elsevier Ltd All rights reserved

引用

页码：9575 / 9585

页数：11

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