Structure of the biological repeating unit of the O-antigen of Pseudomonas aeruginosa immunotype 4 containing both 2-acetamido-2,6-dideoxy-D-glucose and 2-acetamido-2,6-dideoxy-D-galactose

被引：11

作者：

Bystrova, OV

Lindner, B

Moll, H

Kocharova, NA

Knirel, YA ^{[1
]}

Zähringer, U

Pier, GB

机构：

[1] Russian Acad Sci, ND Zelinskii Organ Chem Inst, Moscow 119991, Russia

[2] Res Ctr Borstel, Ctr Med & Biosci, D-23845 Borstel, Germany

[3] Harvard Univ, Sch Med, Brigham & Womens Hosp, Channing Lab, Boston, MA 02115 USA

来源：

CARBOHYDRATE RESEARCH | 2003年 / 338卷 / 17期

关键词：

lipopolysaccharide; O-polysaccharide; O-antigen; repeating unit; Pseudontonas aeruginosa;

D O I：

10.1016/S0008-6215(03)00262-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A phosphorylated core-lipid A backbone oligosaccharide that carries a disaccharide remainder of the first O-antigen repeating unit was derived by strong alkaline degradation following mild hydrazinolysis of the lipopolysaccharide of Pseudomonas aeruginosa immunotype 4 (serogroup O-1). The structure of the oligosaccharide was determined using ESI MS and NMR spectroscopy and it was demonstrated that 2-acetamido-2,6-dideoxy-D-glucose is the first monosaccharide of the O-polysaccharide that is linked to the LPS core. These data define the structure of the biological repeating unit of the O-antigen. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：1801 / 1806

页数：6

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