Rapid identification of Streptococcus thermophilus by primer-specific PCR amplification based on its lacZ gene

被引：63

作者：

Lick, S ^{[1
]}

Keller, M ^{[1
]}

Bockelmann, W ^{[1
]}

Heller, KJ ^{[1
]}

机构：

[1] INST MOL BIOTECHNOL,ABT MOL EVOLUT BIOL,D-07745 JENA,GERMANY

来源：

SYSTEMATIC AND APPLIED MICROBIOLOGY | 1996年 / 19卷 / 01期

关键词：

Streptococcus thermophilus; PCR; lacZ gene; yoghurt;

D O I：

10.1016/S0723-2020(96)80012-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A method is presented allowing rapid and accurate identification of Streptococcus thermophilus by a primer-specific PCR-technique based on its lacZ gene sequence. For subsequent confirmation of the PCR-product (968 bp in length), the assay can additionally be combined with a hybridization step involving a S. thermophilus specific oligonucleotide probe (Lick and Teuber, 1992, System, Appl. Microbiol. 15, 456-459) hybridizing to the amplified sequence. The PCR-assay can be applied for rapid detection of S. thermophilus in fermented milk products like yoghurt. Nonspecific amplification was not observed with DNA's from a number of closely related species and a number of lactic acid bacteria which are used in combination with S. thermophilus for the manufacture of dairy products. This assay, therefore, may be applied for fast species-specific identification of S. thermophilus with no further need for hybridization.

引用

页码：74 / 77

页数：4