Critical role of multidimensional flow cytometry in detecting occult leptomeningeal disease in newly diagnosed aggressive B-cell lymphomas

被引：37

作者：

Di Noto, R. ^{[1
,2
]}

Scalia, G. ^{[1
,2
]}

Abate, G. ^{[1
,2
]}

Gorrese, M. ^{[1
,2
]}

Pascariello, C. ^{[1
,2
]}

Raia, M. ^{[1
,2
]}

Morabito, P. ^{[3
]}

Capone, F. ^{[3
]}

Lo Pardo, C. ^{[3
]}

Mirabelli, P. ^{[1
,2
]}

Mariotti, E. ^{[1
,2
]}

Del Vecchio, L. ^{[1
,2
]}

机构：

[1] Univ Naples Federico 2, Dipartimento Biochim & Biotecnol Med, I-80145 Naples, Italy

[2] CEINGE Biotecnol Avanzate, I-80145 Naples, Italy

[3] AOR A Cardarelli, Serv Immunoematol & Med Trasfus, Naples, Italy

来源：

LEUKEMIA RESEARCH | 2008年 / 32卷 / 08期

关键词：

cerebrospinal fluid; flow cytometry; non-Hodgkin lymphoma;

D O I：

10.1016/j.leukres.2007.12.016

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Among histological aggressive non-Hodgkin lymphomas (NHL), the overall risk of central nervous system (CNS) relapse is approximately 5%, a figure which is too low to offer prophylaxis to all patients. The aim of this work is to demonstrate the utility of flow cytometry (FCM) in detecting occult leptomeningeal disease in this subtype of NHL. We studied cerebrospinal fluid (CSF) involvement in 42 newly diagnosed aggressive NHL patients at risk for CNS involvement. We used multicolour FCM to detect CSF infiltrating neoplastic cells. Among the 42 patients studied, 11 had CSF involvement as detected by FCM. Of these, only four were also positive for conventional morphology (p = 0.046). These results designate that FCM as the first choice technique in NHL CSF clinical cell analysis. (C) 2007 Elsevier Ltd. All rights reserved.

引用

页码：1196 / 1199

页数：4

共 15 条

[1]

CSF flow cytometry greatly improves diagnostic accuracy in CNS hematologic malignancies [J].

Bromberg, J. E. C. ;

Breems, D. A. ;

Kraan, J. ;

Bikker, G. ;

van der Holt, B. ;

Smitt, P. Sillevis ;

van den Bent, M. J. ;

van't Veer, M. ;

Gratama, J. W. .

NEUROLOGY, 2007, 68 (20) :1674-1679

[2]

Flow cytometry analysis of acute promyelocytic leukemia: the power of 'surface hematology' [J].

Di Noto, R. ;

Mirabelli, P. ;

Del Vecchio, L. .

LEUKEMIA, 2007, 21 (01) :4-8

[3]

Enhanced detection of malignant lymphoma in cerebrospinal fluid by multiparameter flow cytometry [J].

Finn, WG ;

Peterson, LC ;

James, C ;

Goolsby, CL .

AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 1998, 110 (03) :341-346

[4]

French CA, 2000, DIAGN CYTOPATHOL, V23, P369, DOI 10.1002/1097-0339(200012)23:6<369::AID-DC1>3.0.CO

[5]

2-3

[6]

High incidence of occult leptomeningeal disease detected by flow cytometry in newly diagnosed aggressive B-cell lymphomas at risk for central nervous system involvement: the role of flow cytometry versus cytology [J].

Hegde, U ;

Filie, A ;

Little, RF ;

Janik, JE ;

Grant, N ;

Steinberg, SM ;

Dunleavy, K ;

Jaffe, ES ;

Abati, A ;

Stetler-Stevenson, M ;

Wilson, WH .

BLOOD, 2005, 105 (02) :496-502

[7]

CNS prophylaxis in lymphoma: Who to target and what therapy to use [J].

Hill, Quentin A. ;

Owen, Roger G. .

BLOOD REVIEWS, 2006, 20 (06) :319-332

[8]

Central nervous system involvement following diagnosis of non-Hodgkin's lymphoma: a risk model [J].

Hollender, A ;

Kvaloy, S ;

Nome, O ;

Skovlund, E ;

Lote, K ;

Holte, H .

ANNALS OF ONCOLOGY, 2002, 13 (07) :1099-1107

[9]

Detection of malignant haematopoietic cells in the cerebrospinal fluid by conventional cytology and flow cytometry [J].

Nückel, H ;

Novotny, JR ;

Noppeney, R ;

Savidou, I ;

Dührsen, U .

CLINICAL AND LABORATORY HAEMATOLOGY, 2006, 28 (01) :22-29

[10]

Lymphoid and myeloid neoplasms involving cerebrospinal fluid: Comparison of morphologic examination and immunophenotyping by flow cytometry [J].

Roma, AA ;

Garcia, A ;

Avagnina, A ;

Rescia, C ;

Elsner, B .

DIAGNOSTIC CYTOPATHOLOGY, 2002, 27 (05) :271-275

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