Phylogenetic analysis of family 6 glycoside hydrolases

被引:16
作者
Mertz, B [1 ]
Kuczenski, RS [1 ]
Larsen, RT [1 ]
Hill, AD [1 ]
Reilly, PJ [1 ]
机构
[1] Iowa State Univ Sci & Technol, Dept Biol & Chem Engn, Ames, IA 50011 USA
关键词
cellobiohydrolase; endoglucanase; glycoside hydrolase family 6; multiple sequence alignment; phylogenetic tree;
D O I
10.1002/bip.20347
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Multiple sequence alignment separates members of glycoside hydrolase Family 6 into eight subfamilies: one of mainly actinobacterial endoglucanases (EGs), one of ascomycotal EGs, one of chytridiomycotal EGs and cellobiohydrolases (CBHs), one of actinobacterial and proteobacterial CBHs, one of chytridiomycotal CBHs, two of ascomycotal CBHs, and one of basidiomycotal CBHs. Each also has some proteins of unknown function. Multiple sequence alignment also extends to all of Family 6 the observation that lengths of loops that form the active-site tunnel in CBHs vary among subfamilies, and along with loop conformations, determine enzyme junction. (c) 2005 Wiley Periodicals, Inc.
引用
收藏
页码:197 / 206
页数:10
相关论文
共 21 条
[11]   ENHANCEMENT OF THE ENDO-BETA-1,4-GLUCANASE ACTIVITY OF AN EXOCELLOBIOHYDROLASE BY DELETION OF A SURFACE LOOP [J].
MEINKE, A ;
DAMUDE, HG ;
TOMME, P ;
KWAN, E ;
KILBURN, DG ;
MILLER, RC ;
WARREN, RAJ ;
GILKES, NR .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (09) :4383-4386
[12]   3-DIMENSIONAL STRUCTURE OF CELLOBIOHYDROLASE-II FROM TRICHODERMA-REESEI [J].
ROUVINEN, J ;
BERGFORS, T ;
TEERI, T ;
KNOWLES, JKC ;
JONES, TA .
SCIENCE, 1990, 249 (4967) :380-386
[13]   THE USE OF CONSERVED CELLULASE FAMILY-SPECIFIC SEQUENCES TO CLONE CELLULASE HOMOLOG CDNAS FROM FUSARIUM-OXYSPORUM [J].
SHEPPARD, PO ;
GRANT, FJ ;
OORT, PJ ;
SPRECHER, CA ;
FOSTER, DC ;
HAGEN, FS ;
UPSHALL, A ;
MCKNIGHT, GL ;
OHARA, PJ .
GENE, 1994, 150 (01) :163-167
[14]   CRYSTAL-STRUCTURE OF THE CATALYTIC DOMAIN OF A THERMOPHILIC ENDOCELLULASE [J].
SPEZIO, M ;
WILSON, DB ;
KARPLUS, PA .
BIOCHEMISTRY, 1993, 32 (38) :9906-9916
[15]   The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools [J].
Thompson, JD ;
Gibson, TJ ;
Plewniak, F ;
Jeanmougin, F ;
Higgins, DG .
NUCLEIC ACIDS RESEARCH, 1997, 25 (24) :4876-4882
[16]   A comparative analysis of two cDNA clones of the cellulase gene family from anaerobic fungus Piromyces rhizinflata [J].
Tsai, CF ;
Qiu, X ;
Liu, JH .
ANAEROBE, 2003, 9 (03) :131-140
[17]   Structural changes of the active site tunnel of Humicola insolens cellobiohydrolase, Cel6A, upon oligosaccharide binding [J].
Varrot, A ;
Schülein, M ;
Davies, GJ .
BIOCHEMISTRY, 1999, 38 (28) :8884-8891
[18]   Structure of the Humicola insolens cellobiohydrolase Cel6A D416A mutant in complex with a non-hydrolysable substrate analogue, methyl cellobiosyl-4-thio-β-cellobioside, at 1.9 Å [J].
Varrot, A ;
Frandsen, TP ;
Driguez, H ;
Davies, GJ .
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2002, 58 :2201-2204
[19]   Crystal structure of the catalytic core domain of the family 6 cellobiohydrolase II, Cel6A, from Humicola insolens, at 1.92 Å resolution [J].
Varrot, A ;
Hastrup, S ;
Schülein, M ;
Davies, GJ .
BIOCHEMICAL JOURNAL, 1999, 337 :297-304
[20]   Mycobacterium tuberculosis strains possess functional cellulases [J].
Varroto, A ;
Leydier, S ;
Pell, G ;
Macdonald, JM ;
Stick, RV ;
Henrissat, B ;
Gilbert, HJ ;
Davies, GJ .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (21) :20181-20184