Properties of phenoloxidase isolated from the cephalothorax of kuruma prawn (Penaeus japonicus)

被引：79

作者：

Benjakul, S ^{[1
]}

Visessanguan, W

Tanaka, M

机构：

[1] Prince Songkla Univ, Fac Agroind, Dept Food Technol, Hat Yai 90112, Songkhla, Thailand

[2] Natl Sci & Technol Dev Agcy, Natl Ctr Genet Engn & Biotechnol, Klongluang 12120, Pathumthani, Thailand

[3] Tokyo Univ Marine Sci & Technol, Dept Food Sci & Technol, Minato Ku, Tokyo 1088477, Japan

来源：

JOURNAL OF FOOD BIOCHEMISTRY | 2005年 / 29卷 / 05期

关键词：

D O I：

10.1111/j.1745-4514.2005.00042.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Phenoloxidase (PO) from the kuruma prawn cephalothorax was partially purified and characterized. The enzyme showed maximal activity at pH 6.5 and 35C. It was stable in a wide pH range of 3-10 but unstable at a temperature greater than 50C. On the basis of activity staining with L-beta-(3,4-dihydroxylphenyl)alanine, the apparent molecular weight was estimated to be 160 kDa. Sodium dodecyl sulfate, methanol and trypsin showed no effect on PO activity, suggesting that the enzyme was isolated in the active form. Phenylthiourea, a copper-chelating agent, and cysteine exhibited the inhibitory activity against PO in a concentration-dependent manner. However, copper acetate addition had no influence on the activity.

引用

页码：470 / 485

页数：16

共 25 条

[1]

Purification and characterization of prophenoloxidase from kuruma prawn Penaeus japonicus [J].