Gene expression profiles in human non-small and small-cell lung cancers

被引：55

作者：

Difilippantonio, S

Chen, Y

Pietas, A

Schlüns, K

Pacyna-Gengelbach, M

Deutschmann, N

Padilla-Nash, HM

Ried, T

Petersen, I

机构：

[1] Univ Hosp Charite, Inst Pathol, D-10098 Berlin, Germany

[2] NCI, Dept Genet, NIH, Bethesda, MD 20892 USA

来源：

EUROPEAN JOURNAL OF CANCER | 2003年 / 39卷 / 13期

关键词：

gene expression profile; suppression subtractive hybridisation; human non-small cell lung cancer; small-cell lung cancer; sequencing; gene mapping and full-length cDNA;

D O I：

10.1016/S0959-8049(03)00419-2

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Suppression subtractive hybridisation (SSH) was performed comparing normal bronchial epithelial cells with a lung squamous cell carcinoma (SCC) and a metastatic small-cell lung carcinoma (SCLC). The sequence analysis of four cDNA libraries revealed 869 individual sequences. Of these, 342 were tested using northern blots of lung cancer cell lines representing the three major subtypes (SCC, adenocarcinoma, SCLC) which confirmed the differential expression of 236 cDNAs. The extended analysis of 31 randomly chosen fragments confirmed the validity of the approach to identify genes associated with lung cancer development. Additionally, five novel full-length cDNA were isolated encoding the microtubule-associated proteins 1A/1B light chain 3, the epithelial V-like antigen 1 (EVA1), the GTP-binding protein SAR1, a new member of the S100-type calcium binding protein family and a new homeobox-containing gene. (C) 2003 Published by Elsevier Science Ltd.

引用

页码：1936 / 1947

页数：12

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