Surface-enhanced Raman spectroscopy of DNA

被引：446

作者：

Barhoumi, Aoune ^{[1
,4
]}

Zhang, Dongmao ^{[2
,4
]}

Tam, Felicia ^{[3
,4
]}

Halas, Naomi J. ^{[1
,2
,4
]}

机构：

[1] Rice Univ, Dept Chem, Houston, TX 77005 USA

[2] Rice Univ, Dept Elect & Comp Engn, Houston, TX 77005 USA

[3] Rice Univ, Dept Phys & Astron, Houston, TX 77005 USA

[4] Rice Univ, Lab Nanophoton, Houston, TX 77005 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2008年 / 130卷 / 16期

关键词：

D O I：

10.1021/ja800023j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report a method for obtaining highly reproducible surface-enhanced Raman spectroscopy (SERS) of single and double-stranded thiolated DNA oligomers. Following a protocol that relaxes the DNA into an extended conformation, SERS spectra of DNA oligonucleoltides are found to be extremely similar, strongly dominated by the Stokes modes of adenine. A spectral correlation function analysis useful for assessing reproducibility and for quantifying the highly complex changes corresponding to modifications in molecular conformation of the adsorbate molecules is introduced. This approach is used to monitor the interaction of DNA with cisplatin, a chemotherapy agent in widespread use.

引用

页码：5523 / 5529

页数：7

共 39 条

[1] Surface-enhanced Raman spectroscopy (SERS) for sub-micromolar detection of DNA/RNA mononucleotides [J].

Bell, Steven E. J. ;

Sirimuthu, Narayana M. S. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (49) :15580-15581

[2] Chiral discrimination in platinum anticancer drugs [J].

Benedetti, M ;

Malina, J ;

Kasparkova, J ;

Brabec, V ;

Natile, G .

ENVIRONMENTAL HEALTH PERSPECTIVES, 2002, 110 :779-782

[3] DNA directed protein immobilization on mixed ssDNA/oligo(ethylene glycol) self-assembled monolayers for sensitive biosensors [J].

Boozer, C ;

Ladd, J ;

Chen, SF ;

Yu, Q ;

Homola, J ;

Jiang, SY .

ANALYTICAL CHEMISTRY, 2004, 76 (23) :6967-6972

[4] RAMAN-SCATTERING FROM NUCLEIC-ACIDS ADSORBED AT A SILVER ELECTRODE [J].

BRABEC, V ;

NIKI, K .

BIOPHYSICAL CHEMISTRY, 1985, 23 (1-2) :63-70

[5] A fluorescence-based method for determining the surface coverage and hybridization efficiency of thiol-capped oligonucleotides bound to gold thin films and nanoparticles [J].

Demers, LM ;

Mirkin, CA ;

Mucic, RC ;

Reynolds, RA ;

Letsinger, RL ;

Elghanian, R ;

Viswanadham, G .

ANALYTICAL CHEMISTRY, 2000, 72 (22) :5535-5541

[6] A DNA microarray system for forensic SNP analysis [J].

Divne, AM ;

Allen, M .

FORENSIC SCIENCE INTERNATIONAL, 2005, 154 (2-3) :111-121

[7] Tip-enhanced Raman spectra of picomole quantities of DNA nucleobases at Au(111) [J].

Domke, Katrin F. ;

Zhang, Dai ;

Pettinger, Bruno .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (21) :6708-+

[8] Sodium and chlorine ions as part of the DNA solvation shell [J].

Feig, M ;

Pettitt, BM .

BIOPHYSICAL JOURNAL, 1999, 77 (04) :1769-1781

[9] Oligonucleotide adsorption to gold nanoparticles: A surface-enhanced raman spectroscopy study of intrinsically bent DNA [J].

Gearheart, LA ;

Ploehn, HJ ;

Murphy, CJ .

JOURNAL OF PHYSICAL CHEMISTRY B, 2001, 105 (50) :12609-12615

[10] Interaction of anticancer drug cisplatin with guanine: Density functional theory and surface-enhanced Raman spectroscopy study [J].

Giese, B ;

McNaughton, D .

BIOPOLYMERS, 2003, 72 (06) :472-489

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