Spheroid-based engineering of a human vasculature in mice

被引：44

作者：

Alajati, Abdullah ^{[1
,2
,3
]}

Laib, Anna M. ^{[1
]}

Weber, Holger ^{[2
]}

Boos, Anja M. ^{[1
,2
]}

Bartol, Arne ^{[1
]}

Ikenberg, Kristian ^{[2
]}

Korff, Thomas ^{[2
]}

Zentgraf, Hanswalter ^{[4
]}

Obodozie, Cynthia ^{[2
]}

Graeser, Ralph ^{[5
]}

Christian, Sven ^{[1
,2
]}

Finkenzeller, Gunter ^{[3
]}

Stark, G. Bjoern ^{[3
]}

Heroult, Melanie ^{[1
,2
]}

Augustin, Hellmut G. ^{[1
,2
]}

机构：

[1] Heidelberg Univ, Med Fac Mannheim CBTM, Joint Res Div Vasc Biol, D-68167 Mannheim, Germany

[2] Tumor Biol Ctr, Dept Vasc Biol & Angiogenesis Res, D-79108 Freiburg, Germany

[3] Univ Freiburg, Med Ctr, Dept Plast & Hand Surg, D-79106 Freiburg, Germany

[4] German Canc Res Ctr, DKFZ, Electron Microscopy Grp, D-69120 Heidelberg, Germany

[5] ProQinase GmbH, D-79108 Freiburg, Germany

来源：

NATURE METHODS | 2008年 / 5卷 / 05期

关键词：

D O I：

10.1038/NMETH.1198

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The complexity of the angiogenic cascade limits cellular approaches to studying angiogenic endothelial cells (ECs). In turn, in vivo assays do not allow the analysis of the distinct cellular behavior of ECs during angiogenesis. Here we show that ECs can be grafted as spheroids into a matrix to give rise to a complex three-dimensional network of human neovessels in mice. The grafted vasculature matures and is connected to the mouse circulation. The assay is highly versatile and facilitates numerous applications including studies of the effects of different cytokines on angiogenesis. Modifications make it possible to study human lymphangiogenic processes in vivo. EC spheroids can also be coimplanted with other cell types for tissue engineering purposes.

引用

页码：439 / 445

页数：7

共 30 条

[1]

Lymphangiogenesis in development and human disease [J].

Alitalo, K ;

Tammela, T ;

Petrova, TV .

NATURE, 2005, 438 (7070) :946-953

[2]

Differential in vivo potential of endothelial progenitor cells from human umbilical cord blood and adult peripheral blood to form functional long-lasting vessels [J].

Au, Patrick ;

Daheron, Laurence M. ;

Duda, Dan G. ;

Cohen, Kenneth S. ;

Tyrrell, James A. ;

Lanning, Ryan M. ;

Fukumura, Dai ;

Scadden, David T. ;

Jain, Rakesh K. .

BLOOD, 2008, 111 (03) :1302-1305

[3]

AUSPRUNK DH, 1975, AM J PATHOL, V79, P597

[4]

Co-culture of bone marrow fibroblasts and endothelial cells on modified polycaprolactone substrates for enhanced potentials in bone tissue engineering [J].

Choong, Cleo S. N. ;

Hutmacher, Dietmar W. ;

Triffitt, James T. .

TISSUE ENGINEERING, 2006, 12 (09) :2521-2531

[5]

Drevs J, 2000, CANCER RES, V60, P4819

[6]

Development of technologies aiding large-tissue engineering [J].

Eiselt, P ;

Kim, BS ;

Chacko, B ;

Isenberg, B ;

Peters, MC ;

Greene, KG ;

Roland, WD ;

Loebsack, AB ;

Burg, KJL ;

Culberson, C ;

Halberstadt, CR ;

Holder, WD ;

Mooney, DJ .

BIOTECHNOLOGY PROGRESS, 1998, 14 (01) :134-140

[7]

Induction, differentiation, and remodeling of blood vessels after transplantation of Bcl-2-transduced endothelial cells [J].

Enis, DR ;

Shepherd, BR ;

Wang, YN ;

Qasim, A ;

Shanahan, CM ;

Weissberg, PL ;

Kashgarian, M ;

Pober, JS ;

Schechner, JS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (02) :425-430

[8]

VEGF and the quest for tumour angiogenesis factors [J].

Ferrara, N .

NATURE REVIEWS CANCER, 2002, 2 (10) :795-803

[9]

Engineering tumors with 3D scaffolds [J].

Fischbach, Claudia ;

Chen, Ruth ;

Matsumoto, Takuya ;

Schmelzle, Tobias ;

Brugge, Joan S. ;

Polverini, Peter J. ;

Mooney, David J. .

NATURE METHODS, 2007, 4 (10) :855-860

[10]

A macroporous hydrogel for the coculture of neural progenitor and endothelial cells to form functional vascular networks in vivo [J].

Ford, MC ;

Bertram, JP ;

Hynes, SR ;

Michaud, M ;

Li, Q ;

Young, M ;

Segal, SS ;

Madri, JA ;

Lavik, EB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (08) :2512-2517

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