The role of cystine knots in collagen folding and stability, part I.: Conformational properties of (Pro-Hyp-Gly)5 and (Pro-(4S)-FPro-Gly)5 model trimers with an artificial cystine knot

被引:39
作者
Barth, D [1 ]
Musiol, H [1 ]
Schütt, M [1 ]
Fiori, S [1 ]
Milbradt, AG [1 ]
Renner, C [1 ]
Moroder, L [1 ]
机构
[1] Max Planck Inst Biochem, D-82152 Martinsried, Germany
关键词
collagen; cystine knot; peptides; protein models; triple helix;
D O I
10.1002/chem.200304917
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
In analogy to the cystine knots present in natural collagens, a simplified disulfide cross-link was used to analyse the conformational effects of a C-terminal artificial cystine knot on the folding of collagenous peptides consisting of solely (Pro-Hyp-Gly) repeating units. Assembly of the a chains into a heterotrimer by previously applied regioselective disulfide-bridging strategies failed because of the high tendency of (Pro-Hyp-Gly), peptides to self-associate and form homotrimers. Only when side-chain-protected peptides were used, for example in the Hyp(tBu) form, and a new protection scheme was adopted, selective interchain-disulfide crosslinking into the heterotrimer in organic solvents was successful. This unexpected strong effect of the conformational properties on the efficiency of well-established reactions was further supported by replacing the Hyp residues with (4S)-fluoroproline, which is known to destabilise triple-helical structures. With the related [Pro-(4S)-FPro-GlY](5) peptides, assembly of the heterotrimer in aqueous solution proceeded in a satisfactory manner. Both the intermediates and the final fluorinated heterotrimer are fully unfolded in aqueous solution even at 4degreesC. Conversely, the disulfide-crossbridged (Pro-Hyp-GlY)(5) heterotrimer forms a very stable triple helix. The observation that thermal unfolding leads to scrambling of the disulfide bridges was unexpected. Although NMR experiments support an extension of the triple helix into the cystine knot, thermolysis is not associated with the unfolding process. In fact, the unstructured fluorinated trimer undergoes an equally facile thermodegradation associated with the intrinsic tendency of unsymmetrical disulfides to disproportionate into symmetrical disulfides under favourable conditions. The experimental results obtained with the model peptides fully support the role of triplehelix nucleation and stabilisation by the artificial cystine knot as previously suggested for the natural cystine knots in collagens.
引用
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页码:3692 / 3702
页数:11
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