Organotypic 3D cell culture models: using the rotating wall vessel to study host-pathogen interactions

被引：235

作者：

Barrila, Jennifer ^{[1
]}

Radtke, Andrea L. ^{[1
]}

Crabbe, Aurelie ^{[1
]}

Sarker, Shameema F. ^{[1
]}

Herbst-Kralovetz, Melissa M. ^{[1
,2
]}

Ott, C. Mark ^{[3
]}

Nickerson, Cheryl A. ^{[1
]}

机构：

[1] Arizona State Univ, Sch Life Sci, Biodesign Inst, Ctr Infect Dis & Vaccinol, Tempe, AZ 85287 USA

[2] Univ Arizona, Coll Med Phoenix, Dept Basic Med Sci, Phoenix, AZ 85004 USA

[3] NASA, Habitabil & Environm Factors Div, Lyndon B Johnson Space Ctr, Houston, TX 77058 USA

来源：

NATURE REVIEWS MICROBIOLOGY | 2010年 / 8卷 / 11期

基金：

美国国家航空航天局; 美国国家卫生研究院;

关键词：

ENTERICA SEROVAR TYPHIMURIUM; EPITHELIAL-CELLS; SIMULATED MICROGRAVITY; INNATE IMMUNITY; IN-VITRO; INTESTINAL EPITHELIUM; 3-DIMENSIONAL CULTURE; EXTRACELLULAR-MATRIX; SALMONELLA-TYPHIMURIUM; TISSUE ARCHITECTURE;

D O I：

10.1038/nrmicro2423

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学];

摘要：

Appropriately simulating the three-dimensional (3D) environment in which tissues normally develop and function is crucial for engineering in vitro models that can be used for the meaningful dissection of host-pathogen interactions. This Review highlights how the rotating wall vessel bioreactor has been used to establish 3D hierarchical models that range in complexity from a single cell type to multicellular co-culture models that recapitulate the 3D architecture of tissues observed in vivo. The application of these models to the study of infectious diseases is discussed.

引用

页码：791 / 801

页数：11

共 115 条

[1]

Cell culture: Biology's new dimension [J].