Ethidium monoazide and propidium monoazide for elimination of unspecific DNA background in quantitative universal real-time PCR

被引：36

作者：

Hein, Ingeborg ^{[1
]}

Schneeweiss, Wilfried ^{[2
]}

Stanek, Christian ^{[2
]}

Wagner, Martin ^{[1
]}

机构：

[1] Univ Vet Med, Inst Milk Hyg Milk Technol & Food Sci, Dept Vet Publ Hlth & Food Sci, A-1210 Vienna, Austria

[2] Univ Vet Med, Dept Small Anim & Horses, Clin Orthopaed Ungulates, A-1210 Vienna, Austria

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2007年 / 71卷 / 03期

关键词：

universal real-time PCR; unspecific DNA background; EMA; PMA;

D O I：

10.1016/j.mimet.2007.09.005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Unspecific background DNA in quantitative universal real-time PCR utilizing a hydrolysis probe was completely suppressed by the addition of EMA or PMA to the PCR mix via cross-linking of the dyes to DNA during 650 W visible light exposure. The proposed procedure had no effect on the sensitivity of the real-time PCR reaction. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：336 / 339

页数：4

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