Multiple displacement amplification prior to single nucleotide polymorphism genotyping in epidemiologic studies

被引:34
作者
Tranah, GJ
Lescault, PJ
Hunter, DJ
De Vivo, I [1 ]
机构
[1] Harvard Univ, Sch Publ Hlth, Dept Epidemiol, Boston, MA 02115 USA
[2] Harvard Univ, Harvard Ctr Canc Prevent, Sch Publ Hlth, Boston, MA 02115 USA
[3] Brigham & Womens Hosp, Dept Med, Channing Lab, Boston, MA 02115 USA
[4] Harvard Univ, Sch Med, Boston, MA 02115 USA
关键词
genotype; multiple displacement amplification; polymorphism; single nucleotide; Taq Mar; whole genome amplification;
D O I
10.1023/A:1024173909401
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We assessed the whole genome amplification strategy, known as multiple displacement amplification (MDA), for use with the TaqMan genotyping platform for DNA samples derived from two case-control studies nested in the Nurses' Health Study and the Physicians' Health Study. Our objectives were to ( 1) quantify DNA yield from samples of varying starting concentrations and ( 2) assess whether MDA products give an accurate representation of the original genomic sequence. Multiple displacement amplification yielded a mean 23 000-fold increase in DNA quantity and genotyping results demonstrate 99.95% accuracy across six SNPs from four genes for 352 samples included in this study. These results suggest that MDA will provide a sufficiently robust amplification of limiting samples of genomic DNA that can be used for SNP genotyping in large case-control studies of complex diseases.
引用
收藏
页码:1031 / 1036
页数:6
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