Multiple displacement amplification prior to single nucleotide polymorphism genotyping in epidemiologic studies

被引：34

作者：

Tranah, GJ

Lescault, PJ

Hunter, DJ

De Vivo, I ^{[1
]}

机构：

[1] Harvard Univ, Sch Publ Hlth, Dept Epidemiol, Boston, MA 02115 USA

[2] Harvard Univ, Harvard Ctr Canc Prevent, Sch Publ Hlth, Boston, MA 02115 USA

[3] Brigham & Womens Hosp, Dept Med, Channing Lab, Boston, MA 02115 USA

[4] Harvard Univ, Sch Med, Boston, MA 02115 USA

来源：

BIOTECHNOLOGY LETTERS | 2003年 / 25卷 / 13期

关键词：

genotype; multiple displacement amplification; polymorphism; single nucleotide; Taq Mar; whole genome amplification;

D O I：

10.1023/A:1024173909401

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We assessed the whole genome amplification strategy, known as multiple displacement amplification (MDA), for use with the TaqMan genotyping platform for DNA samples derived from two case-control studies nested in the Nurses' Health Study and the Physicians' Health Study. Our objectives were to ( 1) quantify DNA yield from samples of varying starting concentrations and ( 2) assess whether MDA products give an accurate representation of the original genomic sequence. Multiple displacement amplification yielded a mean 23 000-fold increase in DNA quantity and genotyping results demonstrate 99.95% accuracy across six SNPs from four genes for 352 samples included in this study. These results suggest that MDA will provide a sufficiently robust amplification of limiting samples of genomic DNA that can be used for SNP genotyping in large case-control studies of complex diseases.

引用

页码：1031 / 1036

页数：6

共 12 条

[11] DEGENERATE OLIGONUCLEOTIDE-PRIMED PCR - GENERAL AMPLIFICATION OF TARGET DNA BY A SINGLE DEGENERATE PRIMER [J].