Adsorption of recombinant human bone morphogenetic protein rhBMP-2m onto hydroxyapatite

被引：81

作者：

Boix, T

Gómez-Morales, J

Torrent-Burgués, J

Monfort, A

Pulgdomènech, P

Rodríguez-Clemente, R

机构：

[1] Univ Politecn Cataluna, Dept Enginyeria Quim, Terrassa 08222, Barcelona, Spain

[2] CSIC, Inst Ciencia Mat Barcelona, Bellaterra 08193, Spain

[3] CSIC, Cid, Inst Biol Mol Barcelona, ES-08034 Barcelona, Spain

[4] Univ Granada, CSIC, IACT, Lab Estudios Cristalog, Granada 18100, Spain

[5] IRTA, Dept Genet Vegetal, Cabrils 08348, Spain

[6] Delegac CSIC Andalucia, Seville 41002, Spain

来源：

JOURNAL OF INORGANIC BIOCHEMISTRY | 2005年 / 99卷 / 05期

关键词：

rhBMP-2m; hydroxyapatite; adsorption; Langmuir isotherm;

D O I：

10.1016/j.jinorgbio.2005.01.011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recombinant human mature bone morphogenetic protein 2 (rhBMP-2m) has been expressed to study its adsorption onto precipitated hydroxyapatite (HA). The influence on the adsorption process of different parameters such as pH and concentrations of calcium, phosphate or NaCl has been investigated. Although the adsorption proceeds rapidly at the initial stages, the maximum adsorbed amount is reached after four hours. The process is notably influenced by adding calcium or phosphate to the system but, while calcium ions increase the adsorption of rhBMP-2m onto HA, phosphate ions inhibit it. The influence of pH and NaCl concentration are notable but less important than those of calcium and phosphate. The adsorption data fit well to a Langmuir adsorption isotherm. The values of the isotherm parameters have been calculated and discussed, and an adsorption mechanism has been proposed. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：1043 / 1050

页数：8

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