Primary structure deduction and molecular modelling from a cDNA of a cellobiohydrolase-like protein from the white-rot fungus Coriolus versicolor

被引：6

作者：

Novo, C ^{[1
]}

Simoes, F ^{[1
]}

Mendenca, D ^{[1
]}

Matos, J ^{[1
]}

Clemente, A ^{[1
]}

机构：

[1] UTPAM, DB, INETI, P-1649038 Lisbon, Portugal

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2001年 / 28卷 / 04期

关键词：

cellobiohydrolase; coriolus versicolor; homology modelling;

D O I：

10.1016/S0141-8130(01)00122-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Molecular cloning and cDNA sequencing analysis were used to elucidate the primary structure of a cellulase-like structure from the white-rot fungus Coriolus versicolor. The cDNA of interest was isolated from a cDNA library obtained from C. versicolor mycelia grown on cellulase inducer medium. A pattern search showed that this cellulase belongs to the glycosyl hydrolases family 6. From the deduced amino acid sequence, models of the binding and catalytic domains were built by homology modelling. The constructed models present a typical cellulose-binding domain at the N-terminal region, a rich Pro, Ser. Thr linker peptide, and a catalytic domain at the C-terminus region. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：285 / 292

页数：8

共 23 条

[1]

The Protein Data Bank [J].