Tissue engineering based on cell sheet technology

被引:350
作者
Matsuda, Noriaki [1 ]
Shimizu, Tatsuya [1 ]
Yamato, Masayuki [1 ]
Okano, Teruo [1 ]
机构
[1] Tokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, Shinjuku Ku, Tokyo 1628666, Japan
关键词
D O I
10.1002/adma.200701978
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Cell sheet technology enables novel approaches to tissue engineering without the use of biodegradable scaffolds. Cell sheet technology consists of a temperature-responsive culture dish, which enables reversible cell adhesion to and detachment from the dish surface by controllable hydrophobicity of the surface. This allows for a non-invasive harvest of cultured cells as an intact monolayer cell sheet including deposited extra cellular matrices. The monolayer cell sheet can be transplanted to host tissues without using biodegradable scaffolds and sutures. Thick tissue constructs and patterned cell sheets using two or more kinds of cell source are also developed by means of layered cell sheets in vitro. This Progress Report summarizes temperature-controlled cell adhesion-detachment behavior and applications of the cell sheet technology to regeneration of cornea, periodontal ligament, bladder epithelia, oesophageal epithelia, myocardium, and liver.
引用
收藏
页码:3089 / 3099
页数:11
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