MbtH-Like Proteins as Integral Components of Bacterial Nonribosomal Peptide Synthetases

被引：133

作者：

Felnagle, Elizabeth A. ^{[1
]}

Barkei, John J. ^{[1
]}

Park, Hyunjun ^{[1
]}

Podevels, Angela M. ^{[1
]}

McMahon, Matthew D. ^{[1
]}

Drott, Donald W. ^{[1
]}

Thomas, Michael G. ^{[1
]}

机构：

[1] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA

来源：

BIOCHEMISTRY | 2010年 / 49卷 / 41期

基金：

美国国家卫生研究院;

关键词：

COLI ENTEROBACTIN SYNTHETASE; BIOSYNTHETIC GENE-CLUSTER; ESCHERICHIA-COLI; ENTF; IDENTIFICATION; ENZYME;

D O I：

10.1021/bi1012854

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The biosynthesis of many natural products of clinical interest involves large, multidomain enzymes called nonribosomal peptide synthetases (NRPSs). In bacteria, many of the gene clusters coding for NRPSs also code for a member of the MbtH-like protein superfamily, which are small proteins of unknown function. Using MbtH-like proteins from three separate NRPS systems, we show that these proteins copurify with the NRPSs and influence amino acid activation. As a consequence, MbtH-like proteins are integral components of NRPSs.

引用

页码：8815 / 8817

页数：3

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