Cationic lipid-DNA complexes in gene delivery:: from biophysics to biological applications

被引:294
作者
de Lima, MCP [1 ]
Simoes, S
Pires, P
Faneca, H
Düzgünes, N
机构
[1] Univ Coimbra, Dept Biochem, P-3000 Coimbra, Portugal
[2] Univ Coimbra, Ctr Neurosci & Cell Biol, P-3000 Coimbra, Portugal
[3] Univ Coimbra, Pharmaceut Technol Lab, Fac Pharm, P-3000 Coimbra, Portugal
[4] Univ Pacific, Sch Dent, Dept Microbiol, San Francisco, CA 94115 USA
关键词
gene therapy; non-viral vectors; lipoplexes; biological barriers;
D O I
10.1016/S0169-409X(01)00110-7
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Great expectations from the application of gene therapy approaches to human disease have been impaired by the unsatisfactory clinical progress observed. Among others, the use of an efficient carrier for nucleic acid-based medicines is considered to be a determinant factor for the successful application of this promising therapeutic strategy. The drawbacks associated with the use of viral vectors, namely those related with safety problems, have prompted investigators to develop alternative methods for gene delivery, cationic lipid-based systems being the most representative. This review focuses on the various parameters that are considered to be crucial to optimize the use of cationic lipid-DNA complexes for gene therapy purposes. Particular emphasis is devoted to the analysis of the different stages involved in the transfection process, from the biophysical aspects underlying the formation of the complexes to the different biological barriers that need to be surpassed for gene expression to occur. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:277 / 294
页数:18
相关论文
共 91 条
  • [31] The role of helper lipids in cationic liposome-mediated gene transfer
    Hui, SW
    Langner, M
    Zhao, YL
    Ross, P
    Hurley, E
    Chan, K
    [J]. BIOPHYSICAL JOURNAL, 1996, 71 (02) : 590 - 599
  • [32] Influence of membrane-active peptides on lipospermine/DNA complex mediated gene transfer
    Kickler, A
    Mechtler, K
    Behr, JP
    Wagner, E
    [J]. BIOCONJUGATE CHEMISTRY, 1997, 8 (02) : 213 - 221
  • [33] Koe G. S., 1997, Pharmaceutical Research (New York), V14, pS57
  • [34] An inverted hexagonal phase of cationic liposome-DNA complexes related to DNA release and delivery
    Koltover, I
    Salditt, T
    Rädler, JO
    Safinya, CR
    [J]. SCIENCE, 1998, 281 (5373) : 78 - 81
  • [36] Liposomes in gene therapy
    Lasic, DD
    Templeton, NS
    [J]. ADVANCED DRUG DELIVERY REVIEWS, 1996, 20 (2-3) : 221 - 266
  • [37] LASIC DD, 1997, LIPOSOMES GENE DELIV, P53
  • [38] Pharmaceutical approach to somatic gene therapy
    Ledley, FD
    [J]. PHARMACEUTICAL RESEARCH, 1996, 13 (11) : 1595 - 1614
  • [39] Detailed analysis of structures and formulations of cationic lipids for efficient gene transfer to the lung
    Lee, ER
    Marshall, J
    Siegel, CS
    Jiang, CW
    Yew, NS
    Nichols, MR
    Nietupski, JB
    Ziegler, RJ
    Lane, MB
    Wang, KX
    Wan, NC
    Scheule, RK
    Harris, DJ
    Smith, AE
    Cheng, SH
    [J]. HUMAN GENE THERAPY, 1996, 7 (14) : 1701 - 1717
  • [40] Characterization of cationic lipid-protamine-DNA (LPD) complexes for intravenous gene delivery
    Li, S
    Rizzo, MA
    Bhattacharya, S
    Huang, L
    [J]. GENE THERAPY, 1998, 5 (07) : 930 - 937