A reporter transgene based on a human keratin 6 gene promoter is specifically expressed in the periderm of mouse embryos

被引：28

作者：

Mazzalupo, S

Coulombe, PA

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Biol Chem, Baltimore, MD 21205 USA

[2] Johns Hopkins Univ, Sch Med, Dept Dermatol, Baltimore, MD 21205 USA

来源：

MECHANISMS OF DEVELOPMENT | 2001年 / 100卷 / 01期

关键词：

skin; keratin; wound repair; periderm; temporary epithelial fusions; beta-galactosidase; transgenic mouse;

D O I：

10.1016/S0925-4773(00)00489-5

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We report the developmental regulation of a lacZ reporter transgene fused to the promoter region of the human keratin 6a gene. In mouse embryos, the transgene is expressed in the periderm (the outermost layer of embryonic epidermis), as are the endogenous keratin 6 alpha and beta genes. A subset of periderm cells, localized to temporary epithelial fusions, is known to contain keratin 6 protein, and we find that these cells also harbor LacZ enzymatic activity. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：65 / 69

页数：5

共 23 条

[1] Periderm cells form cornified cell envelope in their regression process during human epidermal development [J].