Biotechnological applications of green fluorescent protein

被引:119
作者
March, JC
Rao, G
Bentley, WE [1 ]
机构
[1] Univ Maryland, Inst Biotechnol, Ctr Biosyst Res, College Pk, MD 20742 USA
[2] Univ Maryland, Dept Chem Engn, College Pk, MD 20742 USA
[3] Univ Maryland Baltimore Cty, Dept Chem & Biochem Engn, Baltimore, MD 21250 USA
关键词
D O I
10.1007/s00253-003-1339-y
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Since its first use as a reporter gene in 1994, green fluorescent protein (GFP) has served as the researcher's agent: slipping, virtually undetected, into unseen spaces, reporting back valuable information, and securing the delivery of precious cargo through hostile domains. GFPs strength lies in its small size, formidable stability, and relative ease of use. It requires only oxygen and an energy source to do its work, which can be supplied at low cost and high precision, respectively. With such a low threshold for use, GFP is often the first line of inquiry into an unknown space. Here is provided a brief compendium of GFPs contributions to biotechnology. They are linked by a need for a level of information that was previously inaccessible, both spatially and temporally. Protein fusions, transcriptional reporters, whole-organism visualization, and various other screening applications are reviewed with respect to biotechnological applications. Germane molecular improvements to GFP are also discussed.
引用
收藏
页码:303 / 315
页数:13
相关论文
共 203 条
[1]  
Ajjarapu S, 1999, APPL ENVIRON MICROB, V65, P5394
[2]   High throughput studies of gene expression using green fluorescent protein-oxidative stress promoter probe constructs - The potential for living chips [J].
Albano, CR ;
Lu, CH ;
Bentley, WE ;
Rao, G .
JOURNAL OF BIOMOLECULAR SCREENING, 2001, 6 (06) :421-428
[3]  
Albano CR, 1996, BIOTECHNOL TECH, V10, P953
[4]   Green fluorescent protein as a real time quantitative reporter of heterologous protein production [J].
Albano, CR ;
Randers-Eichhorn, L ;
Bentley, WE ;
Rao, G .
BIOTECHNOLOGY PROGRESS, 1998, 14 (02) :351-354
[5]  
Andersen JB, 1998, APPL ENVIRON MICROB, V64, P2240
[6]   gfp-based N-acyl homoserine-lactone sensor systems for detection of bacterial communication [J].
Andersen, JB ;
Heydorn, A ;
Hentzer, M ;
Eberl, L ;
Geisenberger, O ;
Christensen, BB ;
Molin, S ;
Givskov, M .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (02) :575-585
[7]   Construction of a fusion protein between protein A and green fluorescent protein and its application to Western blotting [J].
Aoki, T ;
Takahashi, Y ;
Koch, KS ;
Leffert, HL ;
Watabe, H .
FEBS LETTERS, 1996, 384 (02) :193-197
[8]   Construction of chimeric proteins between protein G and fluorescence-enhanced green fluorescent protein, and their application to immunoassays [J].
Arai, R ;
Ueda, H ;
Nagamune, T .
JOURNAL OF FERMENTATION AND BIOENGINEERING, 1998, 86 (05) :440-445
[9]   Construction of green fluorescent protein reporter genes for genotoxicity test (SOS/umu-Test) and improvement of mutagen-sensitivity [J].
Arai, R ;
Makita, Y ;
Oda, Y ;
Nagamune, T .
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2001, 92 (03) :301-304
[10]   Engineering zucchini yellow mosaic potyvirus as a non-pathogenic vector for expression of heterologous proteins in cucurbits [J].
Arazi, T ;
Slutsky, SG ;
Shiboleth, YM ;
Wang, YZ ;
Rubinstein, M ;
Barak, S ;
Yang, J ;
Gal-On, A .
JOURNAL OF BIOTECHNOLOGY, 2001, 87 (01) :67-82