Antimicrobial susceptibility and occurrence of resistance genes among Salmonella enterica serovar Weltevreden from different countries

被引:104
作者
Aarestrup, FM
Lertworapreecha, M
Evans, MC
Bangtrakulnonth, A
Chalermchaikit, T
Hendriksen, RS
Wegener, HC
机构
[1] Danish Vet Inst, WHO, Collaborating Ctr Antimicrobial Resistance Bacter, DK-1790 Copenhagen V, Denmark
[2] Chulalongkorn Univ, Fac Vet Sci, Ctr Antimicrobial Resistance Monitoring Food Born, Bangkok, Thailand
[3] Natl Inst Hlth, WHO, Int Salmonella & Shigella Ctr,Dept Med Sci, Minist Publ Hlth, Bangkok, Thailand
关键词
Salmonella Weltevreden; antimicrobial resistance; genes; South-East Asia;
D O I
10.1093/jac/dkg426
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Objectives: This study was conducted to investigate the occurrence of antimicrobial resistance among Salmonella Weltevreden isolates from different sources in South-East Asia (Indonesia, Laos, Malaysia, Taiwan, Thailand, Vietnam), Australia, Denmark, New Zealand and the USA. Methods: A total of 503 isolates were examined for susceptibility to antimicrobial agents, and resistant isolates were examined for the presence of selected resistance genes by PCR. Results: Only 48 (9.5%) of the isolates were resistant to one or more of the antimicrobial agents tested. A low frequency of resistance was found towards ampicillin (1.8%), chloramphenicol (1.6%), florphenicol (0.4%), nalidixic acid (1.6%), neomycin (0.6%), streptomycin (4.4%), sulfamethoxazole (4.2%), tetracycline (4.0%) and trimethoprim (1.4%), whereas all isolates were susceptible to co-amoxiclav, ceftiofur, ciprofloxacin, colistin and gentamicin. All nine ampicillin-resistant isolates contained a sequence similar to the bla(TEM-1b) gene, one of the eight chloramphenicol-resistant isolates a sequence similar to the catA1 gene, all three neomycin-resistant isolates a sequence similar to the aphA-2 gene, 16 (73%) of the 22 streptomycin-resistant isolates a sequence similar to the aadA gene, the remaining six (27%) a sequence similar to the strA gene, and all 21 sulfamethoxazole-resistant isolates a sequence similar to the sul2 gene. Thirteen (65%) of the 20 tetracycline-resistant isolates contained the tet(A) gene, four (20%) the tet(B) gene, and one (5%) the tet(C) gene. Conclusions: This study showed a low frequency of resistance among Salmonella Weltevreden isolated from humans and other reservoirs in South-East Asia and elsewhere. There was no major difference in the occurrence of resistance between source or geographical origin.
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页码:715 / 718
页数:4
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