Arabidopsis thaliana MTP1 is a Zn transporter in the vacuolar membrane which mediates Zn detoxification and drives leaf Zn accumulation

被引：236

作者：

Desbrosses-Fonrouge, AG

Voigt, K

Schröder, A

Arrivault, S

Thomine, S

Krämer, U

机构：

[1] Max Planck Inst Mol Plant Physiol, D-14476 Golm, Germany

[2] CNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France

来源：

FEBS LETTERS | 2005年 / 579卷 / 19期

关键词：

cation efflux; intron-spliced hairpin; ZAT; zinc; metal tolerance; hyperaccumulation;

D O I：

10.1016/j.febslet.2005.06.046

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Arabidopsis thaliana metal tolerance protein I (MTP1) of the cation diffusion facilitator family of membrane transport proteins can mediate the detoxification of Zn in Arabidopsis and yeast. Xenopus laevis oocytes expressing AtMTP1 accumulate more Zn than oocytes expressing the AtMTP1(D94A) mutant or water-injected oocytes. An AtMTP1(D94A) fusion protein localizes to the vacuolar membrane in root and leaf cells. The analysis of Arabidopsis transformed with a promoter-GUS construct suggests that AtMTP1 is not produced throughout the plant, but primarily in the subpopulation of dividing, differentiating and expanding cells. RNA interference-mediated silencing of AtMTP1 causes Zn hypersensitivity and a reduction in Zn concentrations in vegetative plant tissues. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：4165 / 4174

页数：10

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[1] Cross-species microarray transcript profiling reveals high constitutive expression of metal homeostasis genes in shoots of the zinc hyperaccumulator Arabidopsis halleri [J].