Respiratory syncytial virus differentially activates murine myeloid and plasmacytoid dendritic cells

被引:32
作者
Boogaard, Ivette
van Oosten, Marijke
van Rijt, Leonie S.
Muskens, Femke
Kimman, Tjeerd G.
Lambrecht, Bart N.
Buisman, Anne-Marie
机构
[1] Natl Inst Publ Hlth & Environm, Lab Vaccine Preventable Dis, NL-3720 BA Bilthoven, Netherlands
[2] Erasmus MC, Dept Pulm & Crit Care Med, Rotterdam, Netherlands
关键词
allergy; dendritic cells; flow cytometry; lung immunology; disease; respiratory syncytial virus;
D O I
10.1111/j.1365-2567.2007.02613.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Respiratory syncytial virus (RSV) is the primary cause of bronchiolitis in young children. Upon infection both T helper 1 (Th1) and Th2 cytokines are produced. Because RSV-induced Th2 responses have been associated with severe immunopathology and aggravation of allergic reactions, the regulation of the immune response following RSV infection is crucial. In this study we examined the influence of RSV on the activation and function of murine bone marrow-derived dendritic cells (DCs). RSV induced the expression of maturation markers on myeloid DCs (mDCs) in vitro. The mDCs stimulated with RSV and ovalbumin (OVA) enhanced proliferation of OVA-specific T cells, which produced both Th1 and Th2 cytokines. In contrast to mDCs, RSV did not induce the expression of maturation markers on plasmacytoid DCs (pDCs), not did it enhance the proliferation of OVA-specific T cells that were cocultured with pDCs. However, RSV stimulated the production of interferon-alpha (IFN-alpha) by pDCs. Our findings indicate a clear difference in the functional activation of DC subsets. RSV-stimulated mDCs may have immunostimulatory effects on both Th1 and Th2 responses, while RSV-stimulated pDCs have direct antiviral activity through the release of IFN-alpha.
引用
收藏
页码:65 / 72
页数:8
相关论文
共 47 条
  • [31] Induction of rapid T cell activation, division, and recirculation by intratracheal injection of dendritic cells in a TCR transgenic model
    Lambrecht, BN
    Pauwels, RA
    Fazekas de St Groth, B
    [J]. JOURNAL OF IMMUNOLOGY, 2000, 164 (06) : 2937 - 2946
  • [32] Myeloid dendritic cells stimulate both Th1 and Th2 immune responses depending on the nature of the antigen
    López, CB
    Fernandez-Sesma, A
    Schulman, JL
    Moran, TM
    [J]. JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, 2001, 21 (09) : 763 - 773
  • [33] An advanced culture method for generating large quantities of highly pure dendritic cells from mouse bone marrow
    Lutz, MB
    Kukutsch, N
    Ogilvie, ALJ
    Rössner, S
    Koch, F
    Romani, N
    Schuler, G
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1999, 223 (01) : 77 - 92
  • [34] Toll-like receptors and innate immunity
    Medzhitov, R
    [J]. NATURE REVIEWS IMMUNOLOGY, 2001, 1 (02) : 135 - 145
  • [35] Respiratory syncytial virus infection in infants is associated with predominant Th-2-like response
    Roman, M
    Calhoun, WJ
    Hinton, KL
    Avendano, LF
    Simon, V
    Escobar, AM
    Gaggero, A
    Diaz, PV
    [J]. AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 1997, 156 (01) : 190 - 195
  • [36] Respiratory syncytial virus fusion protein mediates inhibition of mitogen-induced T-Cell proliferation by contact
    Schlender, J
    Walliser, G
    Fricke, J
    Conzelmann, KK
    [J]. JOURNAL OF VIROLOGY, 2002, 76 (03) : 1163 - 1170
  • [37] Inhibition of toll-like receptor 7- and 9-mediated alpha/beta interferon production in human plasmacytoid dendritic cells by respiratory syncytial virus and measles virus
    Schlender, J
    Hornung, V
    Finke, S
    Günthner-Biller, M
    Marozin, S
    Brzózka, K
    Moghim, S
    Endres, S
    Hartmann, G
    Conzelmann, KK
    [J]. JOURNAL OF VIROLOGY, 2005, 79 (09) : 5507 - 5515
  • [38] Severe respiratory syncytial virus bronchiolitis in infancy and asthma and allergy at age 13
    Sigurs, N
    Gustafsson, PM
    Bjarnason, R
    Lundberg, F
    Schmidt, S
    Sigurbergsson, F
    Kjellman, B
    [J]. AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2005, 171 (02) : 137 - 141
  • [39] Respiratory syncytial virus bronchiolitis in infancy is an important risk factor for asthma and allergy at age 7
    Sigurs, N
    Bjarnason, R
    Sigurbergsson, F
    Kjellman, B
    [J]. AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2000, 161 (05) : 1501 - 1507
  • [40] Plasmacytoid dendritic cells inhibit pulmonary immunopathology and promote clearance of respiratory syncytial virus
    Smit, Joost J.
    Rudd, Brian D.
    Lukacs, Nicholas W.
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 2006, 203 (05) : 1153 - 1159