A nucleosome-free dG-dC-rich sequence element promotes constitutive transcription of the essential yeast RIO1 gene

被引：6

作者：

Angermayr, M ^{[1
]}

Schwerdtfeger, K ^{[1
]}

Bandlow, W ^{[1
]}

机构：

[1] Univ Munich, Dept Biol 1, Bereich Genet, D-80638 Munich, Germany

来源：

BIOLOGICAL CHEMISTRY | 2003年 / 384卷 / 09期

关键词：

chromatin analysis (in vivo footprinting); constitutive promoter; essential gene; gene shuffling; promoter analysis; protein serine kinase; Saccharomyces cerevisiae;

D O I：

10.1515/BC.2003.143

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RIO1 is an essential gene that encodes a protein serine kinase and is transcribed constitutively at a very low level. Transcriptional activation of RIO1 dispenses with a canonical TATA box as well as with classical transactivators or specific DNAbinding factors. Instead, a dGdCrich sequence element, that is located 40 to 48 bp upstream the single site of mRNA initiation, is essential and presumably constitutes the basal promoter. In addition, we demonstrate here that this promoter element comprises a nucleosomefree gap which is centered at the dGdC tract and flanked by two positioned nucleosomes. This element is both, necessary and sufficient, for basal transcription initiation at the RIO1 promoter and, thus, constitutes a novel type of core promoter element.

引用

页码：1287 / 1292

页数：6

共 37 条

[1] Reb1p-dependent DNA bending effects nucleosome positioning and constitutive transcription at the yeast profilin promoter [J].

Angermayr, M ;

Oechsner, U ;

Bandlow, W .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (20) :17918-17926

[2] Permanent nucleosome exclusion from the Ga14p-inducible yeast GCY1 promoter [J].

Angermayr, M ;

Bandlow, W .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (13) :11026-11031

[3] RIO1, an extraordinary novel protein kinase [J].

Angermayr, M ;

Bandlow, W .

FEBS LETTERS, 2002, 524 (1-3) :31-36

[4] The general regulatory factor Reb1p controls basal, but not Gal4p-mediated, transcription of the GCY1 gene in yeast [J].

Angermayr, M ;

Bandlow, W .

MOLECULAR & GENERAL GENETICS, 1997, 256 (06) :682-689

[5] Transcription initiation in vivo without classical transactivators:: DNA kinks flanking the core promoter of the housekeeping yeast adenylate kinase gene, AKY2, position nucleosomes and constitutively activate transcription [J].

Angermayr, M ;

Oechsner, U ;

Gregor, K ;

Schroth, GP ;

Bandlow, W .

NUCLEIC ACIDS RESEARCH, 2002, 30 (19) :4199-4207

[6] Yeast Rio1p is the founding member of a novel subfamily of protein serine kinases involved in the control of cell cycle progression [J].

Angermayr, M ;

Roidl, A ;

Bandlow, W .

MOLECULAR MICROBIOLOGY, 2002, 44 (02) :309-324

[7] The type of basal promoter determines the regulated or constitutive mode of transcription in the common control region of the yeast gene pair GCY1/RIO1 [J].

Angermayr, M ;

Bandlow, W .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (50) :31630-31635

[8] TRANSCRIPTION FACTOR ACCESS IS MEDIATED BY ACCURATELY POSITIONED NUCLEOSOMES ON THE MOUSE MAMMARY-TUMOR VIRUS PROMOTER [J].

ARCHER, TK ;

CORDINGLEY, MG ;

WOLFORD, RG ;

HAGER, GL .

MOLECULAR AND CELLULAR BIOLOGY, 1991, 11 (02) :688-698

[9] ATP-dependent nucleosomere modeling [J].

Becker, PB ;

Hörz, W .

ANNUAL REVIEW OF BIOCHEMISTRY, 2002, 71 :247-273

[10] SATURATION MUTAGENESIS OF A YEAST-HIS3 TATA ELEMENT - GENETIC-EVIDENCE FOR A SPECIFIC TATA-BINDING PROTEIN [J].

CHEN, W ;

STRUHL, K .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (08) :2691-2695

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