Determination of irinotecan (CPT-11) and SN-38 in human whole blood and red blood cells by liquid chromatography with fluorescence detection

被引：39

作者：

de Jong, FA ^{[1
]}

Mathijssen, RHJ ^{[1
]}

de Bruijn, P ^{[1
]}

Loos, WJ ^{[1
]}

Verweij, J ^{[1
]}

Sparreboom, A ^{[1
]}

机构：

[1] Erasmus MC Univ, Med Ctr Rotterdam, Dept Med Oncol, NL-3008 AE Rotterdam, Netherlands

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2003年 / 795卷 / 02期

关键词：

irinotecan; SN-38; CPT-11;

D O I：

10.1016/S1570-0232(03)00574-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An analytical method was developed for the anticancer agent irinotecan (CPT-11) and its main metabolite SN-38 in human whole blood and in red blood cells (RBCs). Sample pretreatment involved deproteinization of whole blood or plasma-diluted RBCs isolated by MESED instruments, with a mixture of aqueous perchloric acid and methanol (1:1, v/v). Separation was carried out using isocratic elution on a Hypersil ODS stationary phase. with detection at excitation and emission wavelengths of 355 and 515 nm, respectively. The lower limit of quantitation (LLQ) in blood was established at 5.00 ng/ml for both compounds, with values for within-run precision (WRP) and between-run precision (BRP) of less than 10%. The method is currently being applied to investigate the blood distribution of CPT-11 and SN-38 in cancer patients. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：383 / 388

页数：6

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