A dual sheath flow cytometer for shipboard analyses of phytoplankton communities from the oligotrophic oceans

被引:25
作者
Cavender-Bares, KK [1 ]
Frankel, SL [1 ]
Chisholm, SW [1 ]
机构
[1] MIT 48425, Ralph M Parsons Lab, Cambridge, MA 02139 USA
关键词
D O I
10.4319/lo.1998.43.6.1383
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Flow cytometry has been used to study phytoplankton since the early 1980s. Because of the wide range of cell sizes and concentrations that occur in natural samples, multiple instrument configurations have been required to characterize pico, ultra-, and nanophytoplankton. Lengthy changeover times between configurations have made synoptic analyses of phytoplankton communities extremely cumbersome and therefore rare. To overcome this problem, we have added dual-sheath capability and adjustable optics to a commercially available flow cytometer. These additions expand the instrument's capabilities during routine operation. The dual-sheath cytometer can characterize both the submicron picoplankter Prochlorococcus at sample flow rates of 10 mu l min(-1) and, after a 1-min changeover, ultraphytoplankton and nanophytoplankton at sample flow rates >1 ml min(-1). These capabilities make possible real-time analysis of phytoplankton size spectra at sea.
引用
收藏
页码:1383 / 1388
页数:6
相关论文
共 41 条
  • [31] DETERMINATION OF THE CROSS-SECTION ABSORPTION-COEFFICIENT OF INDIVIDUAL PHYTOPLANKTON CELLS BY ANALYTICAL FLOW-CYTOMETRY
    PERRY, MJ
    PORTER, SM
    [J]. LIMNOLOGY AND OCEANOGRAPHY, 1989, 34 (08) : 1727 - 1738
  • [32] QUINONESBERGERE.RA, 1992, THESIS DALHOUSIE U, P225
  • [33] CHARACTERIZING AQUATIC BACTERIA ACCORDING TO POPULATION, CELL-SIZE, AND APPARENT DNA CONTENT BY FLOW-CYTOMETRY
    ROBERTSON, BR
    BUTTON, DK
    [J]. CYTOMETRY, 1989, 10 (01): : 70 - 76
  • [34] Shapiro H.M., 1995, Practical Flow Cytometry, V3rd
  • [35] SPATIAL MESOSCALE PATTERNS OF WEST PACIFIC PICOPHYTOPLANKTON AS ANALYZED BY FLOW-CYTOMETRY - THEIR CONTRIBUTION TO SUBSURFACE CHLOROPHYLL MAXIMA
    SHIMADA, A
    HASEGAWA, T
    UMEDA, I
    KADOYA, N
    MARUYAMA, T
    [J]. MARINE BIOLOGY, 1993, 115 (02) : 209 - 215
  • [36] CHLOROPHYLL FLUORESCENCE FROM SINGLE CELLS - INTERPRETATION OF FLOW CYTOMETRIC SIGNALS
    SOSIK, HM
    CHISHOLM, SW
    OLSON, RJ
    [J]. LIMNOLOGY AND OCEANOGRAPHY, 1989, 34 (08) : 1749 - 1761
  • [37] van de Hulst HC, 1957, Light Scattering by Small Particles
  • [38] GROWTH OF PROCHLOROCOCCUS, A PHOTOSYNTHETIC PROKARYOTE, IN THE EQUATORIAL PACIFIC-OCEAN
    VAULOT, D
    MARIE, D
    OLSON, RJ
    CHISHOLM, SW
    [J]. SCIENCE, 1995, 268 (5216) : 1480 - 1482
  • [39] A SIMPLE METHOD TO PRESERVE OCEANIC PHYTOPLANKTON FOR FLOW CYTOMETRIC ANALYSES
    VAULOT, D
    COURTIES, C
    PARTENSKY, F
    [J]. CYTOMETRY, 1989, 10 (05): : 629 - 635
  • [40] FLOW-CYTOMETRY AND CELL SORTING - A TECHNIQUE FOR ANALYSIS AND SORTING OF AQUATIC PARTICLES
    YENTSCH, CM
    HORAN, PK
    MUIRHEAD, K
    DORTCH, Q
    HAUGEN, E
    LEGENDRE, L
    MURPHY, LS
    PERRY, MJ
    PHINNEY, DA
    POMPONI, SA
    SPINRAD, RW
    WOOD, M
    YENTSCH, CS
    ZAHURANEC, BJ
    [J]. LIMNOLOGY AND OCEANOGRAPHY, 1983, 28 (06) : 1275 - 1280