Molecular characterization of porcine picobirnaviruses and development of a specific reverse transcription-PCR assay

被引：32

作者：

Carruyo, Gabriela M. ^{[1
,2
]}

Mateu, Guaniri ^{[1
]}

Martinez, Laura C. ^{[3
]}

Pujol, Flor H. ^{[1
]}

Nates, Silvia V. ^{[3
]}

Liprandi, Ferdinando ^{[1
]}

Ludert, Juan E. ^{[1
]}

机构：

[1] Inst Venezolano Invest Cient, Ctr Microbiol & Biol Celular, Caracas, Venezuela

[2] La Univ Zulia, Fac Vet, Maracaibo, Venezuela

[3] Univ Nacl Cordoba, Fac Ciencias Med, RA-5000 Cordoba, Argentina

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2008年 / 46卷 / 07期

关键词：

D O I：

10.1128/JCM.00655-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The molecular characterization of partial-length genomic segment 2 of porcine picobirnavirus (PBV) strains and the development of a specific reverse transcription-PCR (RT-PCR) assay for detection of virus in feces are reported. Phylogenetic analysis indicated that the studied porcine isolates were more closely related (> 85% identity) to human PBV belonging to genogroup I than to the other porcine PBV described so far. Analysis by RT-PCR and polyacrylamide gel electrophoresis of fecal samples collected in Venezuela and Argentina showed that PBV circulate at high frequencies in piglets.

引用

页码：2402 / 2405

页数：4

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