Electrochemical biosensor for the detection of H2O2 from living cancer cells based on ZnO nanosheets

被引：122

作者：

Rui, Qi ^{[1
]}

Komori, Kikuo ^{[2
]}

Tian, Yang ^{[1
,3
]}

Liu, Haiqing ^{[1
]}

Luo, Yongping ^{[1
]}

Sakai, Yasuyuki ^{[2
]}

机构：

[1] Tongji Univ, Dept Chem, Shanghai 200092, Peoples R China

[2] Univ Tokyo, Inst Ind Sci, Meguro Ku, Tokyo 1538505, Japan

[3] Hunan Univ, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Hunan, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2010年 / 670卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

ZnO nanosheets; Cytochrome c; H2O2; Electrochemical biosensor; Living cells; DIRECT ELECTRON-TRANSFER; NANOPARTICLES-MODIFIED ELECTRODE; HEART CYTOCHROME-C; HYDROGEN-PEROXIDE; PARTICLE-SIZE; HORSERADISH-PEROXIDASE; DIOXIDE NANOPARTICLES; VAPOR-DEPOSITION; THIN-FILMS; IN-VIVO;

D O I：

10.1016/j.aca.2010.04.065

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this work, direct electron transfer of cytochrome c (cyt. c)-a model for studying the electron transfer of enzymes is achieved at hexagonal ZnO nanosheets by one-step electrodeposition. UV-vis spectra and electrochemical data demonstrate that such ZnO nanosheets can supply a bio-compatible surface to keep the bioactivity of cyt. c. The redox formal potential (E-0') of cyt. c is estimated to be 338.2 +/- 4.3 mV (vs. Ag vertical bar AgCl) at the nanostructured ZnO surface. This value is much more positive than those of enzymes previously obtained at other metal oxides and zeolite surfaces. Experiment data show, under the optimized potential of 0.0V (vs. Ag vertical bar AgCl), the electrochemical determination of H2O2 is free from not only anodic interferences like ascorbic acid (AA) and dopamine (DA), but also a cathodic interference-O-2. Such an excellent selectivity enable the present H2O2 biosensor determine the extracellular H2O2 released from living human hepatoma cells. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：57 / 62

页数：6

共 41 条

[1] Vertically aligned sulfur-doped ZnO nanowires synthesized via chemical vapor deposition [J].

Bae, SY ;

Seo, HW ;

Park, JH .

JOURNAL OF PHYSICAL CHEMISTRY B, 2004, 108 (17) :5206-5210

[2] Seeding of colloidal Au nanoparticle solutions. 2. Improved control of particle size and shape [J].

Brown, KR ;

Walter, DG ;

Natan, MJ .

CHEMISTRY OF MATERIALS, 2000, 12 (02) :306-313

[3] ELECTRONIC WAVE-FUNCTIONS IN SEMICONDUCTOR CLUSTERS - EXPERIMENT AND THEORY [J].

BRUS, L .

JOURNAL OF PHYSICAL CHEMISTRY, 1986, 90 (12) :2555-2560

[4] Direct electron transfer of cytochrome c immobilized on a NaY zeolite matrix and its application in biosensing [J].

Dai, ZH ;

Liu, SQ ;

Ju, HX .

ELECTROCHIMICA ACTA, 2004, 49 (13) :2139-2144

[5] Sensor and biosensor based on Prussian Blue modified gold and platinum screen printed electrodes [J].

de Mattos, IL ;

Gorton, L ;

Ruzgas, T .

BIOSENSORS & BIOELECTRONICS, 2003, 18 (2-3) :193-200

[6] In vivo detection of superoxide anion in bean sprout based on ZnO nanodisks with facilitated activity for direct electron transfer of superoxide dismutase [J].

Deng, Zifeng ;

Rui, Qi ;

Yin, Xia ;

Liu, Haiqing ;

Tian, Yang .

ANALYTICAL CHEMISTRY, 2008, 80 (15) :5839-5846

[7] WO3 nanostructures facilitate electron transfer of enzyme: Application to detection of H2O2 with high selectivity [J].

Deng, Zifeng ;

Gong, Yichun ;

Luo, Yongping ;

Tian, Yang .

BIOSENSORS & BIOELECTRONICS, 2009, 24 (08) :2465-2469

[8] ELECTROCHEMISTRY OF HORSE HEART CYTOCHROME-C [J].

EDDOWES, MJ ;

HILL, HAO .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1979, 101 (16) :4461-4464

[9] Electron transfer in self-assembled inorganic polyelectrolyte/metal nanoparticle heterostructures [J].

Feldheim, DL ;

Grabar, KC ;

Natan, MJ ;

Mallouk, TE .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1996, 118 (32) :7640-7641

[10] SURFACE-ENHANCED RESONANCE RAMAN AND ELECTROCHEMICAL INVESTIGATION OF GLUCOSE-OXIDASE CATALYSIS AT A SILVER ELECTRODE [J].

HOLT, RE ;

COTTON, TM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1989, 111 (08) :2815-2821

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