Highly efficient biosynthesis of the oligosaccharide moiety of the GD3 ganglioside by using metabolically engineered Escherichia coli

被引:31
作者
Antoine, T [1 ]
Heyraud, A [1 ]
Bosso, C [1 ]
Samain, E [1 ]
机构
[1] CNRS, CERMAV, F-38041 Grenoble 9, France
关键词
biotechnology; carbohydrates; glycolipids; glycosyltransferases; metabolic engineering;
D O I
10.1002/anie.200461507
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Express order for oligosaccharides: A microbiological process for the synthesis of the carbohydrate portion of gangliosides GD3 and GT3 is described (see structure). Lactose and sialic acid are used as exogenous precursors by a metabolically engineered Escherichia coli strain that overexpresses the bifunctional sialyltransferase cstll gene from Campylobacter jejuni. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码:1350 / 1352
页数:3
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