Highly efficient biosynthesis of the oligosaccharide moiety of the GD3 ganglioside by using metabolically engineered Escherichia coli

被引：31

作者：

Antoine, T ^{[1
]}

Heyraud, A ^{[1
]}

Bosso, C ^{[1
]}

Samain, E ^{[1
]}

机构：

[1] CNRS, CERMAV, F-38041 Grenoble 9, France

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 09期

关键词：

biotechnology; carbohydrates; glycolipids; glycosyltransferases; metabolic engineering;

D O I：

10.1002/anie.200461507

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Express order for oligosaccharides: A microbiological process for the synthesis of the carbohydrate portion of gangliosides GD3 and GT3 is described (see structure). Lactose and sialic acid are used as exogenous precursors by a metabolically engineered Escherichia coli strain that overexpresses the bifunctional sialyltransferase cstll gene from Campylobacter jejuni. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：1350 / 1352

页数：3

共 16 条

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[12] A new fermentation process allows large-scale production of human milk oligosaccharides by metabolically engineered bacteria [J].