Artificial sequence-specific DNA binding peptides: Branched-chain basic regions

被引：17

作者：

Pellegrini, M

Ebright, RH

机构：

[1] RUTGERS STATE UNIV,WAKSMAN INST,NEW BRUNSWICK,NJ 08855

[2] RUTGERS STATE UNIV,DEPT CHEM,NEW BRUNSWICK,NJ 08855

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 1996年 / 118卷 / 25期

关键词：

D O I：

10.1021/ja9528083

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Branched-chain-basic-region peptides (''bcBR'') consisting of two bZIP basic regions connected through a C-terminal (NNepsilon)-N-alpha-lysine linkage bind to DNA with high affinity and high specificity. bcBR with two identical basic regions recognize 2-fold-symmetric DNA sites, and bcBR with two nonidentical basic regions recognize asymmetric DNA sites. Resin-linked bcBR (''bcBR beads''), which are compatible with one-bead/one-peptide combinatorial chemistry approaches, retain high-specificity DNA binding activity and permit high-throughput screening of DNA recognition properties.

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页码：5831 / 5835

页数：5

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