On the general properties of polarised light conventional and confocal microscopes

被引：90

作者：

Torok, P ^{[1
]}

Higdon, PD ^{[1
]}

Wilson, T ^{[1
]}

机构：

[1] Univ Oxford, Dept Engn Sci, Oxford OX1 3PJ, England

来源：

OPTICS COMMUNICATIONS | 1998年 / 148卷 / 4-6期

关键词：

diffraction; high-aperture; focusing; vectorial theory; scanning optical microscopy; confocal microscopy;

D O I：

10.1016/S0030-4018(97)00576-2

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

This paper considers the general properties of polarising microscopes and, in particular, light polarisation changes produced by high aperture lenses. A method is suggested for vectorial ray tracing of complex optical systems and to model high aperture lenses. The two principal arrangements of polarising microscopes (linearly and circularly polarised) are also considered. We discuss the effect of pinhole size on the detected intensity. A theory for point resolution of these microscopes is also presented and results are analysed. We support our theoretical predictions with experimental data. (C) 1998 Elsevier Science B.V.

引用

页码：300 / 315

页数：16

共 17 条

[1] Amperometric glucose sensor based on glucose oxidase immobilized in electrochemically generated poly(ethacridine) [J].