Determination of mycotoxins in bovine milk by liquid chromatography tandem mass spectrometry

被引:175
作者
Sorensen, LK [1 ]
Elbæk, TH [1 ]
机构
[1] Steins Lab, DK-6650 Brorup, Denmark
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2005年 / 820卷 / 02期
关键词
tandem mass spectrometry; milk; mycotoxins; ochratoxin A; trichothecenes; zearalenone; alpha-zearalenol; beta-zearalenol; alpha-zearalanol; zeranol; beta-zearalanol; taleranol; fumonisin B-1; fumonisin B-2; T-2; toxin; HT-2; triol; diacetoxyscirpenol; DAS; monoacetoxyscirpenol; MAS; deoxynivalenol; DON; vomitoxin; 3-acetyldeoxynivalenol; 3-AcDON; 15-acetyldeoxynivalenol; 15-AcDON; deepoxy-deoxynivalenol; DOM-1; aflatoxin M-1;
D O I
10.1016/j.jchromb.2005.03.020
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Liquid chromatographic/tandem mass spectrometric methods using pneumatically assisted electrospray ionisation (LC-ESI-MS/MS) was developed for determination of 18 mycotoxins and metabolites-ochratoxin A, zearalenone, alpha-zearalenol, beta-zearalenol, alpha-zearalanol(zeranol), beta-zearalanol (taleranol), fumonisin B-1, fumonism B-2, T-2 toxin, HT-2 toxin, T-2 triol, diacetoxyscirpenol (DAS), 15-monoacetoxyscirpenol (MAS), deoxynivalenol (DON), 3-acetyideoxynivalenol (3-AcDON), 15-acetyldeoxynivalenol (15-AcDON), deepoxy-deoxynivalenol (DOM-1) and aflatoxin M-1-in milk. The mycotoxins were extracted and cleaned up simultaneously. Extraction and removal of lipophilic compounds was performed at pH 2 using a two-phase mixture of acetonitrile and hexane. The acetonitrile concentration of the aqueous phase was reduced and the pH was adjusted to 8.5 before clean up by solid phase extraction (SPE) on Oasis HLB. The toxins DON, DOM-1, 3-AcDON, 15-AcDON, ochratoxin A, zearalenone, alpha-zearalenol, beta-zearalenol, alpha-zearalanol and beta-zearalanol were detected in negative ion mode after separation on a Hypersil ENV analytical column, while the toxins T-2 toxin, HT-2 toxin, T-2 triol, DAS, MAS, fumonisin B1, famonisin B2 and aflatoxin M, were detected in positive ion mode after separation on a Luna C-18 column. Two transition products were monitored for each compound. The extraction and SPE conditions were optimised to obtain maximum recovery and minimum signal suppression/enhancement. The detection capabilities related to the transition products of lowest abundance were in the range 0.020-0.15 mu g/l. The mean true recoveries were in the range 76-108% at levels of 0.2-10 mu g/l (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:183 / 196
页数:14
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