Construction of high-density bacterial colony arrays and patterns by the ink-jet method

被引：101

作者：

Xu, T

Petridou, S

Lee, EH

Roth, EA

Vyavahare, NR

Hickman, JJ

Boland, T

机构：

[1] Clemson Univ, Dept Bioengn, Clemson, SC 29634 USA

[2] Clemson Univ, Dept Microbiol, Clemson, SC 29634 USA

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 2004年 / 85卷 / 01期

关键词：

ink-jet printer; bacterial colony array; complex pattern; cell density gradient; biosensor;

D O I：

10.1002/bit.10768

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have developed a method for fabricating bacterial colony arrays and complex patterns using commercially available ink-jet printers. Bacterial colony arrays with a density of 100 colonies/cm(2) were obtained by directly ejecting Escherichia coli (E. coli) onto agar-coated substrates at a rapid arraying speed of 880 spots per second. Adjusting the concentration of bacterial suspensions allowed single colonies of viable bacteria to be obtained. In addition, complex patterns of viable bacteria as well as bacteria density gradients were constructed using desktop printers controlled by a simple software program. (C) 2004 Wiley Periodicals, Inc.

引用

页码：29 / 33

页数：5

共 22 条

[1] Microarray sampling-platform fabrication using bubble-jet technology for a biochip system [J].