A MicroRNA Guide for Clinicians and Basic Scientists: Background and Experimental Techniques

被引：80

作者：

Bernardo, Bianca C. ^{[1
]}

Charchar, Fadi J. ^{[2
]}

Lin, Ruby C. Y. ^{[3
]}

McMullen, Julie R. ^{[1
]}

机构：

[1] Baker IDI Heart & Diabet Inst, Melbourne, Vic, Australia

[2] Univ Ballarat, Sch Hlth Sci, Ballarat, Vic 3353, Australia

[3] Univ NSW, Ramaciotti Ctr Gene Funct Anal, Randwick, NSW, Australia

来源：

HEART LUNG AND CIRCULATION | 2012年 / 21卷 / 03期

基金：

澳大利亚研究理事会; 澳大利亚国家健康与医学研究理事会;

关键词：

MicroRNAs; Biomarkers; Research tools; Experimental techniques; Therapeutics; ACUTE MYOCARDIAL-INFARCTION; IN-SITU HYBRIDIZATION; NUCLEIC-ACID PROBES; CIRCULATING MICRORNAS; CARDIAC-HYPERTROPHY; GENE-EXPRESSION; HEART-FAILURE; POTENTIAL BIOMARKER; PERIPHERAL-BLOOD; TISSUE-SECTIONS;

D O I：

10.1016/j.hlc.2011.11.002

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

MicroRNAs (miRNAs) are short non-coding RNA molecules that are approximately 22 nucleotides in length. In the last 10 years, miRNA research and discovery has advanced at a rapid rate. This review provides a brief overview of the discovery and biology of miRNAs, and summarises some of the experimental techniques used for isolation, detection, target prediction, and regulation of miRNAs. We also outline experimental workflows for investigators new to the field, and discuss the diagnostic and therapeutic application of miRNAs. (Heart, Lung and Circulation 2012;21:131-142) (C) 2011 Australian and New Zealand Society of Cardiac and Thoracic Surgeons (ANZSCTS) and the Cardiac Society of Australia and New Zealand (CSANZ). Published by Elsevier Inc. All rights reserved.

引用

页码：131 / 142

页数：12

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