In vivo tracking of human mesenchymal stem cells in experimental stroke

被引：52

作者：

Kim, Daehong ^{[2
]}

Chun, Byoung-Gi ^{[1
]}

Kim, Yeon-Kyung ^{[1
,3
]}

Lee, Yong Hyun ^{[1
,3
]}

Park, Cheong-Soo ^{[2
]}

Jeon, Iksoo ^{[1
]}

Cheong, Chaejoon ^{[2
]}

Hwang, Tae-Sun ^{[1
]}

Chung, Hyungmin ^{[1
,3
]}

Gwag, Byoung Joo ^{[4
]}

Hong, Kwan Soo ^{[2
]}

Song, Jihwan ^{[1
]}

机构：

[1] Pochon CHA Univ, Coll Med, CHA Stem Cell Inst, Seoul 135081, South Korea

[2] Korea Basic Sci Inst, MRI Team, Ochang, South Korea

[3] CHA Biotech Ltd, Stem Cell therapy Inst, Seoul, South Korea

[4] Ajou Univ, Sch Med, Dept Pharmacol, Suwon 441749, Gyeonggi Do, South Korea

来源：

CELL TRANSPLANTATION | 2007年 / 16卷 / 10期

关键词：

migration; human mesenchymal stem cells (hMSCs); middle cerebral artery occlusion (MCAo); magnetic resonance imaging (MRI); MARROW STROMAL CELLS; DIFFERENTIATION; TRANSPLANTATION; RATS; THERAPY; FERUMOXIDES; MIGRATION;

D O I：

10.3727/000000007783472381

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

To understand the fates of human mesenchymal stem cells (hMSCs) following transplantation into a rodent model of middle cerebral artery occlusion (MCAo), magnetic resonance imaging (MRI) techniques were employed. hMSCs were labeled with ferumoxides (Feridex@)-protamine sulfate complexes, which were visualized and examined by MRI up to 10 weeks following transplantation. Migration of the transplanted cells to the infarcted area was further confirmed by histological methods. We found that the hMSCs transplanted in MCAo models possess the capacity to migrate to the infarcted area extensively in both ipsilateral and contralateral injections, exhibiting a pathotropism. We also analyzed the detailed migration patterns of transplanted hMSCs. We speculate that the extensive migratory ability of hMSCs may represent a therapeutic potential for developing efficient cell transplantation strategies in stroke.

引用

页码：1007 / 1012

页数：6

共 21 条

[1] Labeling of cells with ferumoxides-protamine sulfate complexes does not inhibit function or differentiation capacity of hematopoietic or mesenchymal stem cells [J].

Arbab, AS ;

Yocum, GT ;

Rad, AM ;

Khakoo, AY ;

Fellowes, V ;

Read, EJ ;

Frank, JA .

NMR IN BIOMEDICINE, 2005, 18 (08) :553-559

[2] Intracytoplasmic tagging of cells with ferumoxides and transfection agent for cellular magnetic reisonance imaging after cell transplantation: Methods and techniques [J].

Arbab, AS ;

Bashaw, LA ;

Miller, BR ;

Jordan, EK ;

Bulte, JWM ;

Frank, JA .

TRANSPLANTATION, 2003, 76 (07) :1123-1130

[3]

Arbab AS, 2004, BLOOD, V104, P3412

[4] Engraftment and migration of human bone marrow stromal cells implanted in the brains of albino rats - similarities to astrocyte grafts [J].

Azizi, SA ;

Stokes, D ;

Augelli, BJ ;

DiGirolamo, C ;

Prockop, DJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (07) :3908-3913

[5] Autologous mesenchymal stem cell transplantation in stroke patients [J].

Bang, OY ;

Lee, JS ;

Lee, PH ;

Lee, G .

ANNALS OF NEUROLOGY, 2005, 57 (06) :874-882

[6] The surface adhesion molecule CXCR4 stimulates mesenchymal stem cell migration to stromal cell-derived factor-1 in vitro but does not decrease apoptosis under serum deprivation [J].

Bhakta, Shyam ;

Hong, Ping ;

Koc, Omer .

CARDIOVASCULAR REVASCULARIZATION MEDICINE, 2006, 7 (01) :19-24

[7] Magnetodendrimers allow endosomal magnetic labeling and in vivo tracking of stem cells [J].

Bulte, JWM ;

Douglas, T ;

Witwer, B ;

Zhang, SC ;

Strable, E ;

Lewis, BK ;

Zywicke, H ;

Miller, B ;

van Gelderen, P ;

Moskowitz, BM ;

Duncan, ID ;

Frank, JA .

NATURE BIOTECHNOLOGY, 2001, 19 (12) :1141-1147

[8] Mesenchymal stem cells spontaneously express neural proteins in culture and are neurogenic after transplantation [J].

Deng, Jie ;

Petersen, Bryon E. ;

Steindler, Dennis A. ;

Jorgensen, Marda L. ;

Laywell, Eric D. .

STEM CELLS, 2006, 24 (04) :1054-1064

[9] Stem and progenitor cell-based therapy of the human central nervous system [J].

Goldman, S .

NATURE BIOTECHNOLOGY, 2005, 23 (07) :862-871

[10] Monitoring of implanted stem cell migration in vivo:: A highly resolved in vivo magnetic resonance imaging investigation of experimental stroke in rat [J].

Hoehn, M ;

Küstermann, E ;

Blunk, J ;

Wiedermann, D ;

Trapp, T ;

Wecker, S ;

Föcking, M ;

Arnold, H ;

Hescheler, J ;

Fleischmann, BK ;

Schwindt, W ;

Bührle, C .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (25) :16267-16272

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