Oligothiophene phosphoramidites for oligonucleotide labelling

被引：11

作者：

Capobianco, ML ^{[1
]}

Naldi, M ^{[1
]}

Zambianchi, M ^{[1
]}

Barbarella, G ^{[1
]}

机构：

[1] CNR, ISOF, I-40129 Bologna, Italy

来源：

TETRAHEDRON LETTERS | 2005年 / 46卷 / 47期

关键词：

synthesis; oligonucleotides; oligothiophenes; fluorescence; molecular probes;

D O I：

10.1016/j.tetlet.2005.09.123

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

We report the synthesis and characterization of two oligonucleotides (a tetramer and a 19-mer) labelled with a fluorescent oligothiophene and obtained by means of the phosphoramidite of the fluorophore. The conjugate compounds were synthesized in solid phase and characterized by means of mass spectrometry, multinuclear NMR, UV-vis and photoluminescence spectroscopies. The results show that this approach is suitable as a general route for the preparation of oligonucleotides labelled with oligothiophene-based fluorophores. (c) 2005 Published by Elsevier Ltd.

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页码：8181 / 8184

页数：4

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