The Arabidopsis aldehyde oxidase 3 (AA03) gene product catalyzes the final step in abscisic acid biosynthesis in leaves

被引:333
作者
Seo, M
Peeters, AJM
Koiwai, H
Oritani, T
Marion-Poll, A
Zeevaart, JAD
Koornneef, M
Kamiya, Y
Koshiba, T [1 ]
机构
[1] Tokyo Metropolitan Univ, Dept Biol Sci, Hachioji, Tokyo 1920397, Japan
[2] Univ Wageningen & Res Ctr, Genet Lab, NL-6730 HA Wageningen, Netherlands
[3] Tohoku Univ, Grad Sch Agr Sci, Dept Appl Bioorgan Chem, Sendai, Miyagi 9818555, Japan
[4] INRA, Biol Cellulaire Lab, F-78026 Versailles, France
[5] Michigan State Univ, Dept Energy Plant Res Lab, E Lansing, MI 48824 USA
[6] RIKEN, Inst Phys & Chem Res, Wako, Saitama 3510198, Japan
关键词
D O I
10.1073/pnas.220426197
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Abscisic acid (ABA) is a plant hormone involved in seed development and germination and in responses to various environmental stresses. The last step of ABA biosynthesis involves oxidation of abscisic aldehyde, and aldehyde oxidase (EC 1.2.3.1) is thought to catalyze this reaction. An aldehyde oxidase isoform, AO delta, encoded by AAO3, one of four Arabidopsis aldehyde oxidase genes (AAO1, AAO2, AAO3, and AAO4), is the most likely candidate for the enzyme, because it can efficiently catalyze the oxidation of abscisic aldehyde to ABA. Here, we report the isolation and characterization of an ABA-deficient Arabidopsis mutant that maps at the AAO3 locus. The mutant exhibits a wilty phenotype in rosette leaves, but seed dormancy is not affected. ABA levels were significantly reduced in the mutant leaves, explaining the wilty phenotype in rosettes, whereas the level in the mutant seeds was less reduced. No AO delta activity could be detected in the rosette leaves of the mutant. Sequence data showed that the mutant contains a G to A substitution in the AAO3 gene. The mutation causes incorrect splicing of the ninth intron of AAO3 mRNA. We thus conclude that the ABA-deficient mutant is impaired in the AAO3 gene and that the gene product, AO delta, is an aldehyde oxidase that catalyzes the last step of ABA biosynthesis in Arabidopsis, specifically in rosette leaves. Other aldehyde oxidases may be involved in ABA biosynthesis in other organs.
引用
收藏
页码:12908 / 12913
页数:6
相关论文
共 42 条
[11]   THE ISOLATION OF ABSCISIC-ACID (ABA) DEFICIENT MUTANTS BY SELECTION OF INDUCED REVERTANTS IN NON-GERMINATING GIBBERELLIN SENSITIVE LINES OF ARABIDOPSIS-THALIANA (L) HEYNH [J].
KOORNNEEF, M ;
JORNA, ML ;
DERSWAN, DLCB ;
KARSSEN, CM .
THEORETICAL AND APPLIED GENETICS, 1982, 61 (04) :385-393
[12]   The genetic and molecular dissection of abscisic acid biosynthesis and signal transduction in Arabidopsis [J].
Koornneef, M ;
Léon-Kloosterziel, KM ;
Schwartz, SH ;
Zeevaart, JAD .
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 1998, 36 (1-2) :83-89
[13]   Purification and properties of flavin- and molybdenum-containing aldehyde oxidase from coleoptiles of maize [J].
Koshiba, T ;
Saito, E ;
Ono, N ;
Yamamoto, N ;
Sato, M .
PLANT PHYSIOLOGY, 1996, 110 (03) :781-789
[14]   Isolation and characterization of abscisic acid-deficient Arabidopsis mutants at two new loci [J].
LeonKloosterziel, KM ;
Gil, MA ;
Ruijs, GJ ;
Jacobsen, SE ;
Olszewski, NE ;
Schwartz, SH ;
Zeevaart, JAD ;
Koornneef, M .
PLANT JOURNAL, 1996, 10 (04) :655-661
[15]   Arabidopsis mutants with a reduced seed dormancy [J].
LeonKloosterziel, KM ;
vandeBunt, GA ;
Zeevaart, JAD ;
Koornneef, M .
PLANT PHYSIOLOGY, 1996, 110 (01) :233-240
[16]   MOLYBDENUM COFACTOR MUTANTS, SPECIFICALLY IMPAIRED IN XANTHINE DEHYDROGENASE-ACTIVITY AND ABSCISIC-ACID BIOSYNTHESIS, SIMULTANEOUSLY OVEREXPRESS NITRATE REDUCTASE [J].
LEYDECKER, MT ;
MOUREAUX, T ;
KRAEPIEL, Y ;
SCHNORR, K ;
CABOCHE, M .
PLANT PHYSIOLOGY, 1995, 107 (04) :1427-1431
[17]   Molecular biology and regulation of abscisic acid biosynthesis in plants [J].
Liotenberg, S ;
North, H ;
Marion-Poll, A .
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 1999, 37 (05) :341-350
[18]  
Marin E, 1997, PLANT PHYSIOL BIOCH, V35, P369
[19]   Molecular identification of zeaxanthin epoxidase of Nicotiana plumbaginifolia, a gene involved in abscisic acid biosynthesis and corresponding to the ABA locus of Arabidopsis thaliana [J].
Marin, E ;
Nussaume, L ;
Quesada, A ;
Gonneau, M ;
Sotta, B ;
Hugueney, P ;
Frey, A ;
MarionPoll, A .
EMBO JOURNAL, 1996, 15 (10) :2331-2342
[20]  
Mendel RR, 1999, CRIT REV PLANT SCI, V18, P33, DOI 10.1016/S0735-2689(99)00386-X