Fully quantitative imaging of chemical elements in Arabidopsis thaliana tissues using STIM, PIXE and RBS

被引:10
作者
Devès, G
Isaure, MP
Le Lay, P
Bourguignon, J
Ortega, R
机构
[1] Univ Bordeaux 1, CNRS, UMR 5084, F-33175 Gradignan, France
[2] CEA, DRT, LITEN, SAT, F-38054 Grenoble, France
[3] CEA, DSV, DRDC, LPVC, F-38054 Grenoble, France
关键词
scanning transmission ion microscopy; quantitative analysis; biological samples; data analysis;
D O I
10.1016/j.nimb.2005.01.044
中图分类号
TH7 [仪器、仪表];
学科分类号
0804 ; 080401 ; 081102 ;
摘要
An experimental design based on nuclear microprobe techniques has been carried out for a complete characterization of major and trace element contents, and distributions, in biological samples. A new methodology, deriving from the "Q-factor", was developed in order to assess the entire sample composition, including the hydrogen content. This methodology relies on a combination of STIM, mu-PIXE and mu-RBS techniques. The STIM analysis allows the determination of initial sample mass as needed for trace element mass normalization. Then mu-PIXE and mu-RBS analyses were performed simultaneously in order to measure respectively trace elements, carbon, nitrogen and oxygen contents. The variation from the "Q-factor" method lies in the fact that Q was not determined from RBS carbon peak height, because of the hydrogen content dependency of carbon peak height, but from RBS gold signal height as occurring from back-scattering of particles on a thin gold foil placed onto each sample. Consequently the absolute carbon content could be measured, as well as, indirectly, the hydrogen content. The STIM, mu-PIXE and mu-RBS were also used to quantitatively map chemical elements within samples at a sub-cellular scale. Application to the chemical imaging of Arabidopsis thaliana tissues contaminated with caesium will be presented. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:117 / 122
页数:6
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